Fragrance of the rice grain achieved via artificial microRNA-induced down-regulation of OsBADH2
文献类型: 外文期刊
第一作者: Chen, Mingliang
作者: Chen, Mingliang;Wei, Xiangjin;Shao, Gaoneng;Tang, Shaoqing;Luo, Ju;Hu, Peisong;Chen, Mingliang
作者机构:
关键词: fragrant rice;2AP;amiRNA;proline
期刊名称:PLANT BREEDING ( 影响因子:1.832; 五年影响因子:1.956 )
ISSN: 0179-9541
年卷期: 2012 年 131 卷 5 期
页码:
收录情况: SCI
摘要: With 6 figures and 1 table Abstract 2-acetyl-1-pyrroline (2AP) is the principal compound responsible for grain fragrance in rice. In fragrant rice cultivars, BADH2 (betaine-aldehyde dehydrogenase 2) is inactivated. Here, we describe the effect of amiRNA (artificial microRNA) transgenesis targeted at BADH2 in rice. BADH2 (but not BADH1) expression was down-regulated in transgenic lines where the amiRNA was driven by the maize ubiquitin promoter, and in these lines, grain 2AP content was also significantly elevated; meanwhile, 2AP could not be detected in the grain of wild-type lines. The leaf proline content in the transgenics was increased through the simultaneous down-regulation of PRODH and up-regulation of P5CS. In transgenic lines where the same amiRNA was driven by the endosperm-specific promoter GluC, no effect on BADH2 expression was detectable, and the content of grain 2AP did not differ from that present in wild-type grains.
分类号:
- 相关文献
作者其他论文 更多>>
-
Enhancing Folate Content in Japonica Rice Through Co-expression of OsADCS and OsGTPCHI Indica Alleles
作者:Lai, Changkai;Hu, Shikai;Jiao, Guiai;Wang, Ling;Shao, Gaoneng;Zhao, Fengli;Xie, Lihong;Wei, Xiangjin;Lue, Yusong;Sheng, Zhonghua;Tang, Shaoqing;Hu, Peisong;Lai, Changkai;Lai, Changkai
关键词:biofortification; endosperm-specific expression; OsADCS; OsGTPCHI; folate content; grain; Oryza sativa
-
The F-box protein CHALK10 mediates SEMIDWARF-1 ubiquitination and degradation to negatively regulate grain chalkiness in rice
作者:Zhou, Wei;Cao, Ni;Lv, Yusong;Jiao, Guiai;Yu, Junming;Zhu, Maodi;Wu, Jiamin;Zhao, Rumeng;Liu, Yongqiang;Lu, Ao;Hu, Shikai;Wei, Xiangjin;Zhao, Fengli;Tang, Shaoqing;Shao, Gaoneng;Hu, Peisong
关键词:rice; Oryza sativa; CHALK10; SD1; gibberellin; chalkiness; starch metabolism
-
A large-scale gene regulatory network for rice endosperm starch biosynthesis and its application in genetic improvement of rice quality
作者:Huang, Kunyong;Lu, Feifei;Chen, Pengfei;Jiao, Guiai;Zhang, Jian;Zhao, Shaolu;Cao, Ruijie;Shao, Gaoneng;Sheng, Zhonghua;Hu, Shikai;Tang, Shaoqing;Hu, Peisong;Wei, Xiangjin;Lin, Haiyan;Zhao, Shaolu
关键词:starch biosynthesis; transcriptional regulatory network; eating and cooking quality; DNA pull-down; rice
-
OsMYBR1, a 1R-MYB Family Transcription Factor Regulates Starch Biosynthesis in Rice Endosperm
作者:Huang, Kunyong;Chen, Long;Jiao, Guiai;Ruan, Zheyan;Li, Xinwei;Tang, Shaoqing;Hu, Peisong;Wei, Xiangjin;Chen, Long
关键词:rice; starch synthesis; amylose content;
OsMYBR1 ; rice quality -
Production of grains with low glutelin and high eating quality by using dominant allele Lgc-1 in three-line japonica hybrid rice
作者:Hu, Shikai;Yang, Lingwei;Jiao, Guiai;Hui, Suozhen;Zhou, Liang;Cao, Ruijie;Wang, Jingxin;Chen, Yujuan;Fang, Junchao;Sheng, Zhonghua;Tang, Shaoqing;Hu, Peisong;Cai, Jinyang;Yang, Hailong
关键词:rice; low-glutelin restorer line; high eating quality; three-line japonica hybrid rice
-
A novel transcription factor OsMYB73 affects grain size and chalkiness by regulating endosperm storage substances' accumulation-mediated auxin biosynthesis signalling pathway in rice
作者:Liu, Song;Wu, Jiamin;Mawia, Amos Musyoki;Wei, Xiangjin;Cao, Ruijie;Jiao, Guiai;Wu, Yawen;Zhang, Jian;Xie, Lihong;Sheng, Zhonghua;Hu, Shikai;Li, Sanfeng;Lv, Yusong;Lu, Feifei;Chen, Yujuan;Fiaz, Sajid;Tabassum, Javaria;Du, Zhimin;Shao, Gaoneng;Hu, Peisong;Tang, Shaoqing;Liu, Song;Gao, Fangyuan;Ren, Guangjun;Fiaz, Sajid
关键词:
OsMYB73 ; endosperm starch and lipid biosynthesis; auxin biosynthesis; grain filling and endosperm development; grain size and chalkiness; yield and quality -
Intelligent survey method of rice diseases and pests using AR glasses and image-text multimodal fusion model
作者:Chen, Xiangfu;Liu, Yongjian;Wu, Jian;Yao, Qing;Yang, Baojun;Luo, Ju;Liu, Shuhua;Feng, Zelin;Lyu, Jun
关键词:Rice pest and disease; Field survey; Multimodal; RDP-Detector; AR glasses