Identification of in vivo interaction between rabbit hemorrhagic disease virus capsid protein and minor structural protein

文献类型: 外文期刊

第一作者: Yang ZongWei

作者: Yang ZongWei;Chen ZongYan;Li ChuanFeng;Liu GuangQing;Ni Zheng;Yun Tao;Yang ZongWei

作者机构:

关键词: rabbit hemorrhagic disease virus;VP60;VP10;protein-protein interaction

期刊名称:CHINESE SCIENCE BULLETIN ( 影响因子:1.649; 五年影响因子:1.738 )

ISSN: 1001-6538

年卷期: 2012 年 57 卷 30 期

页码:

收录情况: SCI

摘要: We investigated the ability of the rabbit hemorrhagic disease virus (RHDV) capsid protein (VP60) to interact specifically with the minor structural protein VP10, using an in vivo cell-based CheckMate (TM) Mammalian Two-Hybrid System. RHDV VP60 protein interacted specifically with VP10. Immunofluorescence analysis and co-immunoprecipitation with specific antibodies revealed the existence of biologically important VP60/VP10 complexes. However, when VP60 was divided into two fragments, the interaction between VP60 and VP10 was impaired dramatically. These results will be helpful for further investigating the mechanism of RHDV particle assembly.

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