文献类型： 外文期刊

第一作者： Xu Chun-Ying

作者： Xu Chun-Ying;Li Yu-Zhong;Hao Wei-Ping;Li Qiao-Zhen;Dong Yi-Wei;Fang Fu-Li;Guo Zhi-Cheng

作者机构：

关键词： Denitrifier bacterial;Water, nitrate;Nitrogen isotopic composition;Isotope ratio mass spectrometry

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2012 年 40 卷 9 期

页码：

收录情况： SCI

摘要： A method based on denitrifying with TraceGas and Isotope Ratio Mass Spectrometry (IRMS) has been developed for the determination of nitrogen isotopic composition of nitrate in water samples. Bacterial growth, culture conditions and the precision, stability of method were investigated. The sample incubation time and trapping time by TraceGas affecting delta N-15 were examined using standard reference material USGS34. It is very important to obtain bacterial growth success by controlling the volume of oxygen in the culture. The method has good precision and stability with the standard deviations (SD) from 0.09 parts per thousand to 0.14 parts per thousand in the same sample preparation time and 0.12 parts per thousand in 6 months. The values of delta N-15 were almost the same from 3 h to 24 h for the sample incubation time course. 500 s was the best trapping time by TraceGas for the smallest difference between corrected values and real values. The delta N-15 values of nitrate in the livestock wastewater and irrigating water were measured by this method.

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