Upconversion nanoparticles modified with aminosilanes as carriers of DNA vaccine for foot-and-mouth disease
文献类型: 外文期刊
第一作者: Guo, Huichen
作者: Guo, Huichen;Hao, Rongzeng;Sun, Shiqi;Sun, Dehui;Yin, Hong;Liu, Zaixin;Liu, Xiangtao;Guo, Huichen;Hao, Rongzeng;Sun, Shiqi;Sun, Dehui;Yin, Hong;Liu, Zaixin;Liu, Xiangtao;Qian, Haisheng
作者机构:
关键词: Upconversion nanoparticles;Gene delivery;DNA carrier;Foot-and-mouth disease virus;DNA vaccine
期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )
ISSN: 0175-7598
年卷期: 2012 年 95 卷 5 期
页码:
收录情况: SCI
摘要: The potential of the upconversion nanoparticles NaYF4:Yb/Er@silica(UCPs)/plasmid DNA (pcDNA3.1/VP1-GFP) complex in inducing immune responses was evaluated using the UCPs as carriers of the foot-and-mouth disease virus (FMDV AsiaI/Jiangsu2005) DNA vaccine. The UCPs protection against DNaseI degradation was measured using an in vitro inhibition assay. The expression of the plasmid in vivo was determined via confocal microscopy. Its biocompatibility was evaluated through cytotoxicity assay. Based on the results, the aminosilane-modified UCPs can electrostatically bind, condense, and protect plasmid DNA. Cell viability assays demonstrated that the cytotoxicity of the UCPs/plasmid DNA complex is lower than that of the cationic lipid/plasmid DNA complex, and that the transfection efficiency of UCPs is the same as that of the cationic lipid. Furthermore, the UCP/plasmid DNA complex was intramuscularly administered to guinea pigs. Humoral and cellular immune responses were detected using indirect enzyme-linked immunosorbent assay (ELISA), micro-neutralization assay, and T-lymphocyte proliferation assay. Anti-FMDV specific antibodies, neutralizing antibodies, and T-lymphocyte proliferation responses were induced after vaccination. In the challenge test, all the guinea pigs vaccinated with the UCPs/plasmid DNA complex were fully protected from the FMDV challenge. The current study encourages the use of UCPs as an effective nanosystem for gene delivery to cells for in vitro and in vivo vaccination, and other therapeutic applications.
分类号:
- 相关文献
作者其他论文 更多>>
-
Establishment of an indirect ELISA method for detecting bovine coronavirus antibodies based on N protein
作者:Liu, Qiang;Niu, Xiaoxia;Jiang, Lingling;Zhang, Gang;Wang, Pu;Zhang, Sinong;Gao, Weifeng;Guo, Huichen;Wang, Yujiong;Li, Yong;Guo, Huichen
关键词:bovine coronavirus; N protein; eukaryotic expression; indirect ELISA; antibody detection
-
Develop an indirect ELISA utilizing gD protein to detect antibodies against bovine herpesvirus type 1
作者:Liu, Qiang;Niu, Xiaoxia;Jiang, Lingling;Zhang, Gang;Wang, Pu;Zhang, Sinong;Gao, Weifeng;Wang, Yujiong;Guo, Huichen;Li, Yong;Liang, Ruijin;Guo, Huichen
关键词:bovine herpesvirus type 1; gD protein; prokaryotic expression; indirect ELISA; antibody detection
-
Epidemiology, molecular diversity and zoonotic implications of Echinococcus granulosus sensu stricto (G1) in cattle across Nigeria: Evidence of broader transmission dynamics
作者:Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Morenikeji, Olajumoke Abimbola;Odaibo, Georgina Nijideka;Onoja, Bernard Anyebe;Akanbi, Ibikunle Mark;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong
关键词:Echinococcus granulosus sensu lato; Cystic echinococcosis; Nigeria; Genetic diversity; Molecular epidemiology; One health
-
A new intermediate host of Echinococcus shiquicus in Qinghai-Tibet Plateau, China
作者:Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Hou, Xue-Yi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Guo, Min-Kuan;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Shumuye, Nigus Abebe;Gao, Sheng-Zhi;Hou, Xue-Yi;Zhonggecao, Tao;Zhaxilamao, Bao-Quan;Gazangzhuoma, Hong;Doukao, Hong-Bin;Zhang, Fu-Heng;Guo, Min-Kuan;Li, Wen-Dong;Yang, Tao;Fu, Bao-Quan;Yin, Hong;Jia, Wan-Zhong
关键词:Echinococcus shiquicus; Plateau zokor; Intermediate host; Qinghai-Tibet Plateau; Genetic diversity
-
Soybean domestication alters rhizosphere microbial assembly and disrupts the potential bacteria-protist relationships
作者:Zhao, Shaoguan;Zhang, Zhiyang;Ren, Xiangyu;Xiong, Wu;Zhao, Shaoguan;Liu, Chen;Yuan, Ying;Zhang, Zhiyang;Ren, Xiangyu;Yue, Yang;Sun, Shuo;Sun, Shiqi;Zhang, Qi;Xiong, Wu;Shen, Qirong;Zhao, Qingyun;Xing, Guangnan;Xing, Guangnan;Wang, Ming
关键词:Crop domestication; Rhizosphere microbiome; Protist and Bacteria; Generalists and specialists
-
Ferritin and Encapsulin Nanoparticles as Effective Vaccine Delivery Systems: Boosting the Immunogenicity of the African Swine Fever Virus C129R Protein
作者:Zhang, Yue;Ru, Yi;Zhao, Longhe;Hao, Rongzeng;Yang, Yang;Li, Yajun;Zheng, Haixue;Li, Yajun;Zhang, Rong;Jiang, Chenghui;Zheng, Haixue
关键词:nanoparticles; immunogenicity; ferritin; encapsulin; African swine fever virus; targeted lymph nodes; C129R protein
-
Ferritin and Encapsulin Nanoparticles Enhance Immunogenicity of p30 Protein for ASFV Vaccine Development
作者:Zhang, Yue;Zhao, Longhe;Hao, Rongzeng;Yang, Yang;Shen, Chaochao;Shi, Zhengwang;Ru, Yi;Zheng, Haixue;Shen, Chaochao;Shi, Zhengwang;Zheng, Haixue;Zheng, Haixue
关键词:African swine fevervirus; p30 protein; nanoparticles; nanoparticleantigens; immunogenicity