Effects of Different Activation Regimens on Pronuclear Formation and Developmental Competence of In Vitro-Matured Porcine Oocytes After Intracytoplasmic Sperm Injection
文献类型： 外文期刊
第一作者： Cheng, W-M
作者： Cheng, W-M;Wu, Z-H;Zhang, X.;Zhu, Y-B;Pang, Y-W;Guo, M.;Wang, D.;Tian, J-H;Cheng, W-M;Zhang, X.;Wang, D.
作者机构：
期刊名称：REPRODUCTION IN DOMESTIC ANIMALS （ 影响因子：2.005； 五年影响因子：2.154 ）
ISSN： 0936-6768
年卷期： 2012 年 47 卷 4 期
页码：
收录情况： SCI
摘要： Contents To improve the activation protocol for in vitromaturated porcine oocytes after intracytoplasmic sperm injection (ICSI), we examined the combined effect of U0126, a specific inhibitor of mitogen-activated protein kinase kinases, and an electrical pulse on pronuclear formation and developmental competence. Two approaches were tested: (i) 6-h treatment of ICSI oocytes with U0126 applied at different intervals (0, 2, 3 or 4 h) after the electrical pulse and (ii) treatment of ICSI oocytes with U0126 applied 4 h after the electrical pulse over an additional 4, 6 or 8 h. Another protein kinase inhibitor, 6-dimethylaminopurine, was used as a chemical activator in control experiments. The highest rates of diploid embryo formation, normal fertilization and blastocyst formation were observed after 6 h of exposure to U0126 starting 4 h after the electrical pulse. Therefore, U0126 can be used as an activating agent for porcine oocytes fertilized by ICSI.
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