Dual-recognition immune-co-chemical ECL-sensor based on Ti, Mg@N-CDs-induced and novel signal-sensing units Poly (DVB-co-PBA)-reported for alpha-fetoprotein detection

文献类型： 外文期刊

第一作者： Guo, Jianping

作者： Guo, Jianping;Li, Shijie;Wang, Junping;Wang, Junying

作者机构：

关键词： Alpha-fetoprotein; Aminated nano-titanium dioxide; Ti, N/Mg co-doped carbon dots; Poly(DVB-Co-PBA); Immune-co-chemical ECL-assay

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）

ISSN：

年卷期： 2021 年 346 卷

页码：

收录情况： SCI

摘要： In this paper, a high-efficiency electrochemiluminescence (ECL) ternary immune-system was firstly constructed for ultrasensitive assay of alpha-fetoprotein (AFP) based on the Ti, N/Mg co-doped carbon dots (Ti,Mg@N-CDs) as brand-new nanocomposite emitter, Poly(DVB-Co-PBA) as novel AFP-signal reporter and coreaction acceler-ator. A fluorescent microspheres Poly(DVB-Co-PBA), synthesizing based on 4-vinylphenylboronic acid (4-MPBA) and divinylbenzene (DVB), was used as a signal indicator-probe via the specific carbohydrate-AFP interaction to fabricate a dual-recognition label-free immune-co-chemical biosensor. The as-prepared Ti,Mg@N-CDs exhibited high ECL emission of 3.7-folds compared with CDs. Moreover, the large surface area, promising biocompatibility and nitrogen-rich functional group bearing on the nanohybrid was helpful for the antibody adsorption immo-bilization of AntiAFP/Ti,Mg@N-CDs/GCE. The successful synthesis and enhancement mechanism of the nano -materials were also characterized through a serious of techniques including HR-TEM, SEM, EDS, FT-IR, UV-vis, PL and CV. With an immune-co-chemical ECL-assay format, target and signal reporter-probe (Poly(DVB-Co-PBA)) were introduced in sequence via specific reaction. Based on multiple signal amplification and dual recognition strategy, a largely linear detection ranges from 2.25 x 10(-6) to 2250 ng mL(-1) with a low detection limit (1.55 x 10(-7) ng mL-1) were obtained via the resultant ECL ternary (Poly(DVB-Co-PBA)-AFP/AntiAFP/Ti, Mg@N-CDs/GCE) system. This proposed new method was applied to detect human serum samples with satis-factory results and acceptable recovery (96.04 % similar to 111.22 %), indicating a potential application in clinical monitoring of disease biomarkers.

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