BQP35 is a novel member of the intrinsically unstructured protein (IUP) family which is a potential antigen for the sero-diagnosis of Babesia sp BQ1 (Lintan) infection

文献类型： 外文期刊

第一作者： Guan, Guiquan

作者： Guan, Guiquan;Liu, Junlong;Ma, Miling;Liu, Aihong;Niu, Qingli;Li, Youquan;Ren, Qiaoyun;Luo, Jianxun;Yin, Hong;Guan, Guiquan;Moreau, Emmanuelle;Chauvin, Alain;Rogniaux, Helene;Moreau, Emmanuelle

作者机构：

关键词： Babesia sp BQ1 (Lintan) (BQP35);Recombinant protein;Diagnostic antigen;Intrinsically unstructured proteins

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

ISSN： 0304-4017

年卷期： 2012 年 187 卷 3-4 期

页码：

收录情况： SCI

摘要： A new gene of Babesia sp. BQ1 (Lintan) (BQP35) was cloned by screening a merozoite cDNA expression library with infected sheep serum and using rapid amplification of cDNA ends (RACE). The nucleotide sequence of the cDNA was 11 40 bp with an open reading frame (ORF) of 936 bp encoding a 35-kDa predicted polypeptide with 311 amino acid residues. Comparison of BQP35 cDNA and genomic DNA sequences showed that BQP35 does not possess an intron. Recombinant BQP35 (rBQP35), expressed in a prokaryotic expression system, showed abnormally slow migration on SDS-PAGE. Gel shifting, amino acid sequence and in silico disorder region prediction indicated that BQP35 protein has characteristics of intrinsically unstructured proteins (IUPs). This is the first description of such proteins in the Babesia genus. BQP35 induced antibodies production as early as one week after Babesia sp. BQ1 (Lintan) infection in sheep. No cross-reaction was observed with sera from sheep infected with other ovine piroplasms dominant in China, except with Babesia sp. Tianzhu. The interest of BQP35 as a diagnostic antigen is discussed. (C) 2012 Elsevier B.V. All rights reserved.

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