Characterization of three pheromone-binding proteins (PBPs) of Helicoverpa armigera (Hubner) and their binding properties

文献类型： 外文期刊

第一作者： Mei, Xiang-Dong

作者： Mei, Xiang-Dong;Zhang, Yong-Jun;Guo, Yu-Yuan;Zhang, Tian-Tao;Feng, Ji-Nian;Guo, Yu-Yuan;Berg, Bente G.

作者机构：

关键词： Pheromone-binding proteins;Prokaryotic expression;Binding experiment;3-D structure;Helicoverpa armigera

期刊名称：JOURNAL OF INSECT PHYSIOLOGY （ 影响因子：2.354； 五年影响因子：3.045 ）

ISSN： 0022-1910

年卷期： 2012 年 58 卷 7 期

页码：

收录情况： SCI

摘要： Three pheromone-binding proteins of Helicoverpa armigera were cloned and expressed in Escherichia coli. In order to characterize their physiological properties, ligand-binding experiments were performed using five biologically relevant substances including sex pheromones and interspecific signals. The results showed that one of the pheromone-binding proteins, HarmPBP1, binds strongly to each of the two principal pheromone components of H. armigera, (Z)-11-tetradecenal and (Z)-9-hexadecenal, but not to the interspecific signal (Z)-9-tetracecenal. The two remaining pheromone-binding proteins, HarmPBP2 and HarmPBP3, showed only weak affinities with the ligands tested. The 3-D structure of HarmPBP1 was predicted and the docking experiments indicate that the key binding site of (Z)-9-hexadecenal to HarmPBP1 includes Thr112, Lys111, and Phe119 whereas that of (Z)-11-tetradecenal includes Ser9, Trp37, Phe36, and Phe119. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.

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