Purification, crystallization and preliminary X-ray diffraction analysis of the effector protein PevD1 from Verticillium dahliae

文献类型： 外文期刊

第一作者： Han, Lei

作者： Han, Lei;Liu, Zheng;Qiu, Dewen;Liu, Xinqi

作者机构：

期刊名称：ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS （ 影响因子：0.524； 五年影响因子：0.451 ）

ISSN： 1744-3091

年卷期： 2012 年 68 卷

页码：

收录情况： SCI

摘要： The effector protein PevD1 from the pathogenic fungus Verticillium dahliae was purified and crystallized using the hanging-drop vapour-diffusion method. Native crystals appeared in a solution consisting of 4.0 M sodium formate. A native data set was collected at 1.9 angstrom resolution at 100 Kusing an in-house X-ray source. Because of the absence of useful methinione in the protein sequence, derivative crystals that contained iodine were obtained by soaking in 1.25 M potassium iodide, and a data set that contained anomalous signal was collected using the same X-ray facility at a wavelength of 1.54 angstrom. The single-wavelength anomalous dispersion method was used to successfully solve the structure based on the anomalous signal generated from iodine.

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