Rescue of virulent class I Newcastle disease virus variant 9a5b-D5C1

文献类型： 外文期刊

第一作者： Yu, Yang

作者： Yu, Yang;Qiu, Xusheng;Xu, Dan;Zhan, Yuan;Meng, Chunchun;Wei, Nana;Chen, Hongjun;Tan, Lei;Yu, Shengqing;Ding, Chan;Ding, Chan;Yu, Yang;Liu, Xiufan;Qin, Aijian

作者机构：

关键词： Newcastle disease virus;Reverse genetics;Minigenome;Helper plasmids

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2012 年 9 卷

页码：

收录情况： SCI

摘要： Background: The virulent class I Newcastle disease virus (NDV) variant 9a5b was generated from a nonvirulent NDV isolate Goose/Alaska/415/91 via nine consecutive passages in the chicken air sac, followed by five passages in the chick brain. The evolutionary mechanism of virulence in the class I NDV isolate is not fully understood. To elucidate this evolutionary mechanism, a reverse genetics manipulation specific for class I NDV is indispensable. Results: A full-length cDNA clone of 9a5b and the helper plasmids pCI-NP, pCI-P, and pCI-L were constructed from segments of cDNA. After these plasmids were co-transfected into BSR T7/5 cells, infectious viral particles were obtained. The rescued viruses were genetically and biologically identical to the parental strain and showed similar pathogenicity in chickens. Conclusion: A stable recovery method for class I NDV was established. Reverse genetics of the class I NDV variant 9a5b allowed for the generation of genetically altered and virulent NDV, and can be used as a foundation for research on the evolution of virulence in class I NDV isolates.

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