Biological control of bacterial wilt in Arabidopsis thaliana involves abscissic acid signalling

文献类型: 外文期刊

第一作者: Feng, Dong Xin

作者: Feng, Dong Xin;Tasset, Celine;Hanemian, Mathieu;Barlet, Xavier;Tremousaygue, Dominique;Deslandes, Laurent;Marco, Yves;Feng, Dong Xin;Tasset, Celine;Hanemian, Mathieu;Barlet, Xavier;Tremousaygue, Dominique;Deslandes, Laurent;Marco, Yves;Feng, Dong Xin;Hu, Jian

作者机构:

关键词: abscissic acid (ABA);Arabidopsis thaliana;biological control;defense signalling;hrp genes;microarray;Ralstonia solanacearum

期刊名称:NEW PHYTOLOGIST ( 影响因子:10.151; 五年影响因子:10.475 )

ISSN: 0028-646X

年卷期: 2012 年 194 卷 4 期

页码:

收录情况: SCI

摘要: Means to control bacterial wilt caused by the phytopathogenic root bacteria Ralstonia solanacearum are limited. Mutants in a large cluster of genes (hrp) involved in the pathogenicity of R similar to solanacearum were successfully used in a previous study as endophytic biocontrol agents in challenge inoculation experiments on tomato. However, the molecular mechanisms controlling this resistance remained unknown. We developed a protection assay using Arabidopsis thaliana as a model plant and analyzed the events underlying the biological control by genetic, transcriptomic and molecular approaches. High protection rates associated with a significant decrease in the multiplication of R similar to solanacearum were observed in plants pre-inoculated with a similar to hrpB mutant strain. Neither salicylic acid, nor jasmonic acid/ethylene played a role in the establishment of this resistance. Microarray analysis showed that 26% of the up-regulated genes in protected plants are involved in the biosynthesis and signalling of abscissic acid (ABA). In addition 21% of these genes are constitutively expressed in the irregular xylem cellulose synthase mutants (irx), which present a high level of resistance to R similar to solanacearum. We propose that inoculation with the ?hrpB mutant strain generates a hostile environment for subsequent plant colonization by a virulent strain of R similar to solanacearum.

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