Molecular characterization, tissue distribution and expression analysis of Interleukin-12 receptor beta 2 chain in sheep

文献类型： 外文期刊

第一作者： Meng, Xuelian

作者： Meng, Xuelian;Guo, Aijiang;Gong, Wei;Jia, Wanzhong;Luo, Xuenong;Zhai, Junjun;Dou, Yongxi;Cai, Xuepeng

作者机构：

关键词： IL-12R beta 2;Sheep;Molecular characterization;Expression;Tissue distribution

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2012 年 499 卷 1 期

页码：

收录情况： SCI

摘要： Ovine beta 2 subunit of the interleukin (IL)-12 receptor (IL-12R beta 2) was cloned from mRNA preparation of mitogen-activated peripheral blood mononuclear cells (PBMCs). The complete coding sequence for ovine IL-12 R beta 2 was found to be 2586 nucleotides in length encoding 862-amino-acid residue protein. It showed 96.4% homology at the nucleotide level and 94.1% homology at the amino acid level with bovine IL-12 beta 2. The ovine IL-12 R beta 2 subunit shares common structural and functional elements with their counterparts from the other species. Phylogenetic tree showed that ovine IL-12R beta 2 was clustered into the Artiodactyla group, together with those of cattle and pig, which was distinct from the other groups. Real-time RT-PCR was used to investigate expression of the IL-12R beta 2 in different tissues of sheep in order to determine the characterization of this receptor in tissue. Expression analysis showed that IL-12R beta 2 mRNA expression was detected at all the detected tissues with the exception of thymus. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.

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