A rapid mismatch polymerase chain reaction assay to detect carriers of complex vertebral malformation in Holstein cattle

文献类型: 外文期刊

第一作者: Wang, Shuai

作者: Wang, Shuai;Wang, Zongli;Wang, Shuai;Hao, Haisheng;Zhao, Xueming;Zhu, Huabin;Du, Weihua;Wang, Dong;Liu, Yan;Qin, Tong;Wang, Shuai

作者机构:

关键词: Cattle;complex vertebral malformation;polymerase chain reaction mismatch amplification mutation assay;polymerase chain reaction-primer introduced restriction analysis

期刊名称:JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION ( 影响因子:1.279; 五年影响因子:1.522 )

ISSN: 1040-6387

年卷期: 2012 年 24 卷 3 期

页码:

收录情况: SCI

摘要: Three Holstein sires were identified as complex vertebral malformation (CVM) carriers using the polymerase chain reaction-primer introduced restriction analysis (PCR-PIRA) method. Using the carriers as positive controls, the PCR mismatch amplification mutation assay (MAMA PCR) method was developed and validated by sequence analysis. With MAMA PCR, 154 Chinese Holstein sires were tested for CVM, among which 24 were confirmed to be CVM carriers. With DNA isolated from hair follicles, 10 daughters of one CVM-positive bull were detected, among which 7 were confirmed to be CVM carriers.

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