Development of Real-Time Polymerase Chain Reaction for Detection of Borrelia burgdorferi sensu lato in China

文献类型： 外文期刊

第一作者： Fu, Yuguang

作者： Fu, Yuguang;Liu, Zhijie;Guan, Guiquan;Niu, Qingli;Li, Youquan;Yang, Jifei;Ren, Qiaoyun;Ma, Miling;Liu, Aihong;Peng, Yulv;Luo, Jianxun;Yin, Hong

作者机构：

关键词： Borrelia burgdorferi sensu lato;Real-time PCR;Yak and sheep

期刊名称：VECTOR-BORNE AND ZOONOTIC DISEASES （ 影响因子：2.133； 五年影响因子：2.491 ）

ISSN： 1530-3667

年卷期： 2012 年 12 卷 5 期

页码：

收录情况： SCI

摘要： Universal primers and probes were selected on the basis of the 16S rRNA gene sequence of Borrelia burgdorferi in GenBank (R), and a real-time polymerase chain reaction (PCR) method for detection of B. burgdorferi was established. The results showed that this method could specifically detect the B31 strain (Borrelia burgdorferi sensu stricto), the BO23 strain (Borrelia afzelii), and the SZ strain (Borrelia garinii), without cross-reaction with genome DNA of Theileria (T. luwenshuni, T. uilenbergi, T. sinensis, T. annulata, T. sergenti, T. annulata), Babesia (B. bigemina, B. ovate, B. sp. (Xinjiang)), Anaplasma (A. marginale, A. ovis), Mycoplasma mycoides subsp. capri, and Chlamydia psittaci, which are the infective pathogens to yak and/or sheep. The sensitivity of this real-time PCR is 10(4) times greater than that of a conventional PCR. The real-time PCR was able to amplify 16S rRNA gene from as few as 22.88 fg genomic DNA of B. burgdorferi sensu lato. Tick DNAs from 369 field samples collected from Shangzhi City of Heilongjiang Province were tested, resulting in an infection rate of 42.80%, and a total of 332 genomic DNAs from the blood of 186 yaks and 146 sheep in the Gannan Tibetan Autonomous Prefecture of Gansu Province were tested, resulting in 24.19% positive rate for the yaks and 39.04% positive rate for the sheep.

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