A rescued NADC30-like virus by reverse genetic manipulation exhibits moderate virulence and a promising application perspective

文献类型： 外文期刊

第一作者： Cao, Zhengda

作者： Cao, Zhengda;Chen, Jinxia;Li, Liwei;Liu, Jiachen;Tong, Wu;Zhou, Yanjun;Tong, Guangzhi;Gao, Fei;Tong, Wu;Zhou, Yanjun;Tong, Guangzhi;Gao, Fei;Cao, Zhengda;Wang, Guihua;Gao, Fei

作者机构：

关键词： NADC30-like strain; Reverse genetic system; Full-length infectious clone; Pathogenesis

期刊名称：VIRUS RESEARCH （ 影响因子：6.286； 五年影响因子：4.486 ）

ISSN： 0168-1702

年卷期： 2022 年 316 卷

页码：

收录情况： SCI

摘要： NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV), which is highly homologous to the NADC30 strain isolated in the United States. The NADC30-like PRRSV was first reported in 2014 in China, where it spread and gradually caused an epidemic. Currently, growing research has shown that NADC30-like strains have greater propensity to recombine with other PRRSV strains, particularly the PPRSV vaccine virus used clinically, making the prevention and control of PRRSV highly complex. To carry out an in-depth molecular biology and virulence analysis, a full-length infectious clone of the NADC30-like strain was successfully con-structed and rescued by reverse genetic manipulation. The rescued virus, rZJqz, was indistinguishable from its parental virus, ZJqz21, based on virological characteristics. Further animal experiments demonstrated that rZJqz retained similar pathogenicity and induced the typical clinical symptoms and viral shedding observed in the ZJqz21 challenge model. Together, these results provide a useful tool for further study of the biological char-acteristics and pathogenicity of NADC30-like strains. Moreover, these findings also provide a solid foundation for studying the recombination of different PRRSVs and developing new and effective universal vaccines in the future.

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