Phylogenetic studies of Oesophagostomum asperum from goats based on sequences of internal transcribed spacers of ribosomal deoxyribonucleic acid (DNA)

文献类型: 外文期刊

第一作者: Yu, San-Ke

作者: Yu, San-Ke;Hu, Bing;Li, Hong-Mei;Ren, Wan-Xin;Bian, Qing-Qing;Gao, Man;Wang, Xing-Ye;Cong, Mei-Mei;Song, Jun-Ke;Lin, Qing;Zhao, Guang-Hui;Deng, Yan;Yu, San-Ke;Hu, Bing;Deng, Yan;Li, Hong-Mei;Xu, Min-Jun;Zhao, Guang-Hui

作者机构:

关键词: Phylogenetic study;Oesophagostomum asperum;internal transcribed spacer (ITS);Cashmere goat

期刊名称:AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH ( 影响因子:0.539; 五年影响因子:0.564 )

ISSN: 1996-0808

年卷期: 2012 年 6 卷 13 期

页码:

收录情况: SCI

摘要: In the present study, the phylogenetic position of Oesophagostomum asperum was examined based on the sequences of internal transcribed spacer (ITS) of ribosomal DNA. The DNA fragment spanning 3' end of 18S rDNA, complete ITS-1, 5.8S, ITS-2 rDNA, and 5' end of 28S rDNA was amplified from six nematodes morphologically identified as Oesophagostomum asperum from 3 infected Cashmere goats (one pair parasites for each animal) in Shaanxi province, China. The amplicons were sequenced, and compared with corresponding sequences of other Oesophagostomum species available in GenBank (TM). The ITS-1, 5.8S, and ITS-2 sequences of all the parasites were 374, 153 and 259 bp in length, respectively, with no sequence difference in 5.8S rDNA and only very subtle sequence heterogeneity in ITS-1 and ITS-2 rDNA. The interspecies difference in ITS-1 and ITS-2 rDNA between O. asperum and other Oesophagostomum species showed that O. asperum is closely related to O. venulosum. Phylogenetic studies using Bayesian analysis revealed that all the O. asperum isolates clustered together sistered to O. venulosum. Our study characterized the O. asperum at molecular level for the first time, which would have important implications for further molecular identification and differentiation, and the genetic analysis of this nematode between specimens and hosts and the haplotypic variation and within regions in Shaanxi province and other locations.

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