Improving rice yield and quality by QTL pyramiding
文献类型: 外文期刊
第一作者: Wang, Peng
作者: Wang, Peng;Xing, Yongzhong;Yu, Sibin;Wang, Peng;Yu, Sibin;Li, Zhikang;Li, Zhikang
作者机构:
关键词: Pleiotropy;Heading date;Heterogeneous inbred families;Marker-assisted selection;Rice (Oryza sativa L.)
期刊名称:MOLECULAR BREEDING ( 影响因子:2.589; 五年影响因子:2.75 )
ISSN: 1380-3743
年卷期: 2012 年 29 卷 4 期
页码:
收录情况: SCI
摘要: To facilitate marker-assisted transfer of desirable genes for improvement of yield traits, we used a set of backcross recombinant inbred lines (BRIL) derived from two elite parental lines, 'Zhenshan97' and '93-11', to resolve a quantitative trait loci (QTL) cluster for heading date and yield-related traits in rice. Four main-effect QTL (qHD6.1, qHD6.2, qHD7, and qHD8) and four epistatic QTL affecting heading date in the BRIL were detected in two experimental trials. The major QTL (qHD8) was confirmed in three heterogeneous inbred families (HIF) that segregated for this target region, and narrowed down to a 20-kb segment in a large HIF-derived population. qHD8 was found to interact with qHD7 and had a pleiotropic effect responsible for heading date and yield components. To test usability of the identified QTL in rice improvement, we further developed near-isogenic lines (NIL) containing one or more target genes by marker-assisted transfer of '93-11' alleles at qHD8, qHD7, and qHD6.1, and the GS3 gene for grain size into 'Zhenshan97'. The pyramid line NIL(qHD8 ? GS3) had higher yield potential, longer grains, and a more suitable heading date than 'Zhenshan97'. Comparison of the NIL showed existence of epistasis between alleles at different loci and background effect on qHD8, which are very important for pyramiding of desirable alleles at the target QTL. These results will be particularly useful not only to understand the genetic basis of yield-related traits but also to improve the efficiency of marker-assisted selection for favorable loci in rice breeding programs.
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