Effects of methyl bromide fumigation on community structure of denitrifying bacteria with nitrous oxide reductase gene (nosZ) in soil
文献类型: 外文期刊
第一作者: Zhang Teng
作者: Zhang Teng;Li Yuan;Yan Dongdong;Mao Liangang;Wan Qiuxia;Guo Meixia;Cao Aocheng;Yan Pingmei
作者机构:
关键词: Methyl bromide fumigation;denitrifying bacteria;nosZ gene;community structure
期刊名称:AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH ( 影响因子:0.539; 五年影响因子:0.564 )
ISSN: 1996-0808
年卷期: 2012 年 6 卷 9 期
页码:
收录情况: SCI
摘要: Soil fumigation can inevitably affect the activity of non-target soil microorganisms, the effects of the methyl bromide fumigation on community structure and activity of denitrifying bacteria in soil were studied by nosZ-PCR-RFLP (restriction fragment length polymorphism) method. The results showed that Margalef index, Shannon-wiener index and Evenness index of methyl bromide fumigation soil and nosZ -denitrifying bacteria significantly different, the number of denitrifying bacteria in CK soil was 1.4 times higher than soil fumigated through nosZ-MPN-PCR (Most-Probable-Number-PCR), and denitrification activity of soil fumigated by methyl bromide was very significantly higher than the control soil using soil nitrate eradication.
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