Identification of the para-nitrophenol catabolic pathway, and characterization of three enzymes involved in the hydroquinone pathway, in pseudomonas sp 1-7

文献类型： 外文期刊

第一作者： Zhang, Shuangyu

作者： Zhang, Shuangyu;Sun, Wen;Xu, Li;Zheng, Xiaomei;Chu, Xiaoyu;Tian, Jian;Wu, Ningfeng;Fan, Yunliu

作者机构：

关键词： para-Nitrophenol;Catabolism;Hydroquinone pathway;Hydroxyquinol pathway;Pseudomonas

期刊名称：BMC MICROBIOLOGY （ 影响因子：3.605； 五年影响因子：4.283 ）

ISSN： 1471-2180

年卷期： 2012 年 12 卷

页码：

收录情况： SCI

摘要： Background: para-Nitrophenol (PNP), a priority environmental pollutant, is hazardous to humans and animals. However, the information relating to the PNP degradation pathways and their enzymes remain limited. Results: Pseudomonas sp. 1-7 was isolated from methyl parathion (MP)-polluted activated sludge and was shown to degrade PNP. Two different intermediates, hydroquinone (HQ) and 4-nitrocatechol (4-NC) were detected in the catabolism of PNP. This indicated that Pseudomonas sp. 1-7 degraded PNP by two different pathways, namely the HQ pathway, and the hydroxyquinol (BT) pathway (also referred to as the 4-NC pathway). A gene cluster (pdcEDGFCBA) was identified in a 10.6 kb DNA fragment of a fosmid library, which cluster encoded the following enzymes involved in PNP degradation: PNP 4-monooxygenase (PdcA), p-benzoquinone (BQ) reductase (PdcB), hydroxyquinol (BT) 1,2-dioxygenase (PdcC), maleylacetate (MA) reductase (PdcF), 4-hydroxymuconic semialdehyde (4-HS) dehydrogenase (PdcG), and hydroquinone (HQ) 1,2-dioxygenase (PdcDE). Four genes (pdcDEFG) were expressed in E. coli and the purified pdcDE, pdcG and pdcF gene products were shown to convert HQ to 4-HS, 4-HS to MA and MA to beta-ketoadipate respectively by in vitro activity assays. Conclusions: The cloning, sequencing, and characterization of these genes along with the functional PNP degradation studies identified 4-NC, HQ, 4-HS, and MA as intermediates in the degradation pathway of PNP by Pseudomonas sp. 1-7. This is the first conclusive report for both 4-NC and HQ-mediated degradation of PNP by one microorganism.

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