Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals

文献类型： 外文期刊

第一作者： Ren, Li-Yan

作者： Ren, Li-Yan;Zhang, Yong-Jun;Wu, Kong-Ming;Guo, Yu-Yuan;Li, Ke-Ming

作者机构：

关键词： Braconidae;Electroantennogram;Hymenoptera;Microplitis mediator;Odorant receptor;Quantitative real-time polymerase chain reaction (qPCR);Reverse transcription PCR (RT-PCR) analysis;RNA interference (RNAi);Walking/flight behavior;Y-tube bioassay

期刊名称：JOURNAL OF CHEMICAL ECOLOGY （ 影响因子：2.626； 五年影响因子：2.984 ）

ISSN： 0098-0331

年卷期： 2012 年 38 卷 3 期

页码：

收录情况： SCI

摘要： Odorant receptors are thought to play critical roles in the perception of chemosensory stimuli by insects. The primary method to address the functions of odorant receptors in insects is to use in vitro binding assays between the receptors and potential chemical stimuli. We injected MmedOrco dsRNA into the abdominal cavity of a braconid wasp, Microplitis mediator, and assayed for expression of this gene 72 h after treatment (RNAi). Quantitative real-time PCR demonstrated that the level of mRNA expression in MmedOrco dsRNA-treated M. mediator was significantly reduced (> 90%) when compared with water-treated controls. Furthermore, electroantennogram (EAG) responses of M. mediator to two chemical attractants, nonanal and farnesene, were also reduced significantly (similar to 70%) in RNAi-treated M. mediator when compared to controls. RNAi-treated M. mediator also responded by walking/flying at a lower rate to both chemicals when compared with controls in a Y-tube olfactometer bioassay, which provides direct evidence that MmedOrco plays an important role in perception of nonanal and farnesene in M. mediator.

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