High-level expression of the Penicillium notatum glucose oxidase gene in Pichia pastoris using codon optimization

文献类型： 外文期刊

第一作者： Gao, Zhaowei

作者： Gao, Zhaowei;Zhang, Yuhong;Li, Mu;Zhou, Liwei;Zhang, Wei;Gao, Zhaowei;Tang, Yunming;Li, Zhuofu;Huang, Huoqing;Yao, Bin

作者机构：

关键词： Codon optimization;Glucose oxidase;Penicillium notatum;Pichia pastoris

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

ISSN： 0141-5492

年卷期： 2012 年 34 卷 3 期

页码：

收录情况： SCI

摘要： The glucose oxidase (GOD) gene from Penicillium notatum was expressed in Pichia pastoris. The 1,815 bp gene, god-w, encodes 604 amino acids. Recombinant GOD-w had optimal activity at 35-40 degrees C and pH 6.2 and was stable, from pH 3 to 7 maintaining >75% maximum activity after incubation at 50 degrees C for 1 h. GOD-w worked as well as commercial GODs to improve bread making. To achieve high-level expression of recombinant GOD in P. pastoris, 272 nucleotides involving 228 residues were mutated, consistent with the codon bias of P. pastoris. The optimized recombinant GOD-m yielded 615 U ml(-1) (2.5 g protein l(-1)) in a 3 l fermentor-410% higher than GOD-w (148 U ml(-1)), and thus is a low-cost alternative for the bread baking industry.

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