Expression of CYP107Z13 in Streptomyces lividans TK54 catalyzes the oxidation of avermectin to 4aEuro(3)-oxo-avermectin

文献类型： 外文期刊

第一作者： Liu, Weide

作者： Liu, Weide;Ji, Ying;Niu, Jing;Li, Mei

作者机构：

关键词： Expression;CYP107Z13;Oxidation;Avermectin;Streptomyces lividans TK54

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2012 年 93 卷 5 期

页码：

收录情况： SCI

摘要： Streptomyces ahygroscopicus ZB01 has strong catalytic activity for the regiospecific oxidation of 4aEuro(3)-OH of avermectin to form 4aEuro(3)-oxo-avermectin. A cytochrome P450 gene from S. ahygroscopicus ZB01, cyp107z13, was cloned into pKC1139 to generate pKCZ1 and was transformed into Streptomyces lividans TK54, which does not have the ability to catalyze the conversion of avermectin. CYP107Z13, under the control of an ermE* promoter, was actively expressed in the TK54 recombinant strain as determined by a reduced CO difference spectrum analysis of the crude protein. Analysis of whole-cell biocatalytic activity by high-performance liquid chromatography revealed the recombinant to be able to oxidize avermectin regiospecifically to 4aEuro(3)-oxo-avermectin and CYP107Z13 to be a regioselective oxidase of avermectin. In addition, the whole-cell reaction conditions of the recombinant were optimized. Growth on medium ISP-2 at pH 6 was more conducive for the expression of CYP107Z13 than on medium PYG1 or at pH 7, and active cells of the recombinant strain had higher biocatalytic activity than resting cells.

分类号：

  • 相关文献

[1]Fabrication, Characterization, and Biological Activity of Avermectin Nano-delivery Systems with Different Particle Sizes. Wang, Anqi,Wang, Yan,Sun, Changjiao,Wang, Chunxin,Cui, Bo,Zhao, Xiang,Zeng, Zhanghua,Yao, Junwei,Yang, Dongsheng,Liu, Guoqiang,Cui, Haixin,Wang, Anqi,Wang, Yan,Sun, Changjiao,Wang, Chunxin,Cui, Bo,Zhao, Xiang,Zeng, Zhanghua,Yao, Junwei,Yang, Dongsheng,Liu, Guoqiang,Cui, Haixin. 2018

[2]Sensitivity of Bemisia Tabaci (Hemiptera: Aleyrodidae) to Several New Insecticides in China: Effects of Insecticide Type and Whitefly Species, Strain, and Stage. Xie, Wen,Liu, Yang,Wang, Shaoli,Wu, Qingjun,Pan, Huipeng,Yang, Xin,Guo, Litao,Zhang, Youjun. 2014

[3]Synthesis of 4 ''-benzyloxyimino-4 ''-deoxyavermectin B1 a derivatives. Liang, Xiao Mei,Fang, Xue Qin,Wu, Jing Ping,Wang, Dao Quan,Rui, Chang Hui,Fan, Man Lin,Zhao, Hai Yan,Wang, Yun Xia. 2007

[4]Application of combined physicochemical and biological processes for enhanced treatment of avermectin fermentation wastewater. Yu, Anfeng,Huang, PengYu,Feng, Quan,Chu, Libing,Xing, Xin-Hui,Gui, Dawei,Wang, Haisheng. 2009

[5]Sublethal effect of avermectin and acetamiprid on the mortality of different life stages of Brontispa longissima (Gestro) (Coleoptera: Hispidae) and its larvae parasitoid Asecodes hispinarum Boucek (Hymenoptera: Eulophidae). Jin, Tao,Lin, Yu-ying,Jin, Qi-an,Wen, Hai-bo,Peng, Zheng-qiang. 2014

[6]Genotoxicity evaluation of low doses of avermectin to hemocytes of silkworm (Bombyx mori) and response of gene expression to DNA damage. Shen, WeiFeng,Chen, YuYin,Shen, WeiFeng,Niu, BaoLong,Liu, Yan,He, LiHua,Weng, HongBiao,Meng, ZhiQi,Zhao, XuePing,Wang, Qiang. 2011

[7]Relative fitness of avermectin-resistant strain of Neoseiulus cucumeris (Oudemans) (Acari: Phytoseiidae). Chen, Xia,Zhang, Yan-Xuan,Wei, Hui,Lin, Jian-Zhen,Sun, Li,Zhang, Yu-Ping,Wei, Hui,Chen, Feng.

[8]Population Susceptibility to Insecticides and the Development of Resistance in Bactrocera cucurbitae (Diptera: Tephritidae). Jin, Tao,Lin, Yu-Ying,Jin, Qi-An,Wen, Hai-Bo,Peng, Zheng-Qiang.

[9]Synthesis, biological activities and structure-activity relationships for new avermectin analogues. Zhang, Jian,Nan, Xiang,Cheng, Pi-Le,Zhang, Yan,Liu, Ying-Qian,Liu, Huanxiang,Zhang, Shao-Yong,Chen, An-Liang,Yu, Hai-Tao,Hu, Guan-Fang.

[10]The oxidative transformation of sodium arsenite at the interface of alpha-MnO2 and water. Li, Xiu-juan,Liu, Cheng-shuai,Li, Fang-bai,Zhang, Li-jia,Liu, Chuan-ping,Li, Xiu-juan,Zhou, Yong-zhang,Li, Yong-tao,Zhang, Li-jia,Li, Xiu-juan. 2010

[11]Enhancement of tolerance of Indian mustard (Brassica juncea) to mercury by carbon monoxide. Meng, De Kun,Yang, Zhi Min,Chen, Jian. 2011

[12]Oxidation of elemental sulfur in paddy soils as influenced by flooded condition and plant growth in pot experiment. Zhou, W,Wan, M,He, P,Li, ST,Lin, B. 2002

[13]Relationship between oxidative degradation of 2-mercaptobenzothiazole and physicochemical properties of manganese (hydro)oxides. Liu, C. S.,Zhang, L. J.,Wu, C. A.,Li, F. B.,Feng, C. H.,Liu, C. S.,Li, X. Z.. 2009

[14]Mono-oxidation of alpha-aroyl ketene dithioacetals with hydrogen peroxide: an unexpected stereoselectivity. Yu, CY,Wang, LB,Huang, ZT,Hsu, ACT. 1997

[15]Oxidation of (-)-epicatechin is a precursor of litchi pericarp enzymatic browning. Liu, Liang,Xu, Yujuan,Zhang, Mingwei,Xiao, Gengsheng,Cao, Shaoqian,Deng, Qianchun.

[16]Effects of Oxidation on Water Distribution and Physicochemical Properties of Porcine Myofibrillar Protein Gel. Li, Yin,Li, Xia,Wang, Jin-zhi,Zhang, Chun-hui,Sun, Hong-mei,Wang, Chun-qing,Xie, Xiao-lei. 2014

[17]Effects of acute and chronic heat stress on plasma metabolites, hormones and oxidant status in restrictedly fed broiler breeders. Xie, Jingjing,Tang, Li,Lu, Lin,Zhang, Liyang,Luo, Xugang,Tang, Li,Lin, Xi,Liu, Hsiao-Ching,Odle, Jack.

[18]Influence of dietary vitamin E supplementation on meat quality traits and gene expression related to lipid metabolism in the Beijing-you chicken. Li, W. J.,Zhao, G. P.,Chen, J. L.,Zheng, M. Q.,Wen, J..

[19]Effect of Chromium on Oxidation in Wear of Surface Nanocrystalline Martensite Steel. Zhang, Hao,Tang, Youyun,Fu, Licai. 2016

[20]Detoxification of Arsenite through Adsorption and Oxidative Transformation on Pyrolusite. Cao, Weidong,Liu, Chengshuai,Wang, Xiangqing,Li, Xiujuan,Yang, Jinyan,Cao, Weidong. 2012

作者其他论文 更多>>

微信小程序