A Thermophilic Cellulase Complex from Phialophora sp G5 Showing High Capacity in Cellulose Hydrolysis

文献类型： 外文期刊

第一作者： Zhao, Junqi

作者： Zhao, Junqi;Shi, Pengjun;Bai, Yingguo;Huang, Huoqing;Luo, Huiying;Xu, Donghao;Wang, Yaru;Yao, Bin;Zhang, Huitu

作者机构：

关键词： Phialophora sp G5;Thermophilic;Thermostability;Cellulase complex

期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）

ISSN： 0273-2289

年卷期： 2012 年 166 卷 4 期

页码：

收录情况： SCI

摘要： A cellulase-producing mesophilic fungal strain, named G5, was isolated from the acidic wastewater and mud of a tin mine and identified as Phialophora sp. based on the internal transcribed spacer sequence. The volumetric activities and specific activities of cellulase induced by different carbon sources (Avicel, corn cob, wheat bran and corn stover) were compared. The cellulase complex of Phialophora sp. G5 exhibited the optimal activities at 60-65 A degrees C and pH 4.0-5.0, and had good long-term thermostability at 50 A degrees C. Compared with the commercial cellulase (Accellerase 1500, Genencor), the enzyme under study showed 60% and 80% of the capacity to hydrolyze pure cellulose and natural cellulose, respectively. This is the first study to report that a cellulytic enzymes complex from Phialophora genus, and the superior properties of this enzyme complex make strain G5 a potential microbial source to produce cellulase for industrial applications, and the production ability could be improved by mutagenesis.

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