文献类型: 外文期刊
第一作者: Shi, Pengjun
作者: Shi, Pengjun;Qiu, Zhenhua;Bai, Yingguo;Yuan, Tiezheng;Huang, Huoqing;Pan, Xia;Yang, Peilong;Yao, Bin;Zhang, Wei
作者机构:
关键词: Streptomyces megasporus;Xylanase;Pichia pastoris;Xylobiose
期刊名称:WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY ( 影响因子:3.312; 五年影响因子:3.58 )
ISSN: 0959-3993
年卷期: 2012 年 28 卷 2 期
页码:
收录情况: SCI
摘要: A new xylanase gene, xynBM4, was cloned from Streptomyces megasporus DSM 41476 and expressed in Pichia pastoris. The full-length gene consists of 1,443 bp and encodes 480 amino acids including a putative 49-residue signal peptide. The deduced amino acid sequence of xynBM4 shows the highest identity of 66.3% to the xylanase Xys1L from Streptomyces halstedii JM8. The purified recombinant XYNBM4 had a high specific activity of 350.7 U mg(-1) towards soluble wheat arabinoxylan, exhibited optimal activity at pH 6.0 and 57A degrees C, showed broad pH adaptability (> 75% of the maximum activity at pH 2.5-9.0), was resistant to neutral proteases and most chemicals, and produced simple products. The hydrolysis products of birchwood xylan and corncob xylan were predominantly xylobiose (76.9 and 90.8%, respectively) and no xylose. These characteristics suggest that XYNBM4 has potential in various applications, especially in the food industry.
分类号:
- 相关文献
作者其他论文 更多>>
-
Membralin is required for maize development and defines a branch of the endoplasmic reticulum-associated degradation pathway in plants
作者:Liu, Baiyu;Zhang, Ke;Qi, Shoumei;Jin, Zhe;Chen, Donghua;Zhang, Wei;Zhang, Kewei;Li, Kunpeng;Xu, Changzheng;He, Qiuxia;Cheng, Wen;Ding, Zhaohua;Zhao, Xiangyu
关键词:endoplasmic reticulum-associated degradation; maize; membralin; plant development; unfold protein response
-
Biocontrol performance and mass production potential of the larval endoparasitoid Campoletis chlorideae Uchida (Hymenoptera: Ichneumonidae) against the fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
作者:Zhang, Rui;Zhang, Wei;Zhao, Qi;Keyhani, Nemat O.;Lei, Xian-Fu;Liu, Chang-Hua;Al Dhafer, Hathal M.;Mohamed, Amr
关键词:Spodoptera frugiperda; Field investigation; Campoletis chlorideae; Biological control; Mass production; Two-sex life table
-
Construction of a high-density genetic map for yardlong bean and identification of ANT1 as a regulator of anthocyanin biosynthesis
作者:Zhang, Hongmei;Zhang, Wei;Liu, Xiaoqing;Chen, Xin;Chen, Huatao;Meng, Shan;Yan, Wei;Hui, Linchong;Chen, Wei
关键词:
-
Homoeologous exchanges contribute to branch angle variations in rapeseed: Insights from transcriptome, QTL-seq and gene functional analysis
作者:Sun, Chengming;Zhou, Xiaoying;Fu, Sanxiong;Wang, Xiaodong;Peng, Qi;Gao, Jianqin;Chen, Feng;Zhang, Wei;Hu, Maolong;Zhang, Jiefu;Wu, Jian;Liu, Huimin;Wang, Youping;Xue, Zhifei;Fu, Tingdong;Yi, Bin
关键词:Rapeseed; Branch angle; Homoeologous exchange; WGCNA; QTL-seq; WRKY40
-
A genome-wide association analysis for salt tolerance during the soybean germination stage and development of KASP markers
作者:Wang, Junyan;Zhou, Miaomiao;Su, Chengfu;Wang, Junyan;Zhou, Miaomiao;Zhang, Hongmei;Liu, Xiaoqing;Zhang, Wei;Wang, Qiong;Jia, Qianru;Chen, Huatao;Xu, Donghe;Chen, Huatao
关键词:soybean; salt tolerance; germination stage; genome-wide association analysis; KASP marker
-
Urea Coated with Polyaspartic Acid-Chitosan Increases Foxtail Millet (Setaria italica L. Beauv.) Grain Yield by Improving Nitrogen Metabolism
作者:Lu, Lin;Zhang, Wei;Xv, Yanli;Dong, Haosheng;Chen, Disu;Yan, Peng;Dong, Zhiqiang;Wang, Qi;Zhang, Wei;Dong, Zhiqiang;Gao, Ming;Li, Shujie
关键词:fertilizer; nitrate reductase; nitrogen availability; high yield and efficiency; one-time basic fertilizer application
-
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
作者:Li, Lanxue;Bai, Yingguo;Yao, Bin;Luo, Huiying;Tu, Tao;Liu, Xiaoqing
关键词:high-throughput screening; virtual screening; protein engineering; enzyme thermostability