文献类型: 外文期刊
第一作者: Shi, Pengjun
作者: Shi, Pengjun;Qiu, Zhenhua;Bai, Yingguo;Yuan, Tiezheng;Huang, Huoqing;Pan, Xia;Yang, Peilong;Yao, Bin;Zhang, Wei
作者机构:
关键词: Streptomyces megasporus;Xylanase;Pichia pastoris;Xylobiose
期刊名称:WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY ( 影响因子:3.312; 五年影响因子:3.58 )
ISSN: 0959-3993
年卷期: 2012 年 28 卷 2 期
页码:
收录情况: SCI
摘要: A new xylanase gene, xynBM4, was cloned from Streptomyces megasporus DSM 41476 and expressed in Pichia pastoris. The full-length gene consists of 1,443 bp and encodes 480 amino acids including a putative 49-residue signal peptide. The deduced amino acid sequence of xynBM4 shows the highest identity of 66.3% to the xylanase Xys1L from Streptomyces halstedii JM8. The purified recombinant XYNBM4 had a high specific activity of 350.7 U mg(-1) towards soluble wheat arabinoxylan, exhibited optimal activity at pH 6.0 and 57A degrees C, showed broad pH adaptability (> 75% of the maximum activity at pH 2.5-9.0), was resistant to neutral proteases and most chemicals, and produced simple products. The hydrolysis products of birchwood xylan and corncob xylan were predominantly xylobiose (76.9 and 90.8%, respectively) and no xylose. These characteristics suggest that XYNBM4 has potential in various applications, especially in the food industry.
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