Establishment and Biological Characteristics of Qing Kedan Chicken Embryonic Fibroblast Line for Genetic Resource Conservation

文献类型： 外文期刊

第一作者： Wang, Hui

作者： Wang, Hui;Li, Xiangchen;Li, Changli;Zhang, Wenxiu;Guan, Weijun;Ma, Yuehui;Wang, Hui

作者机构：

关键词： Qing Kedan chicken;embryonic fibroblast line;genetic resource conservation;somatic cell cryopreservation;China

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2012 年 11 卷 23 期

页码：

收录情况： SCI

摘要： A fibroblast cell line of Qing Kedan chicken was successfully isolated, purified and cryopreseved through direct culturing of explants, serial passage and cryogenic techniques. Assays for biological characteristics were conducted and the results suggested that: the cells cultured with a Population Doubling Time (PDT) of about 20 h, constituted a typical fibroblast cell line in. morphology; tests for microbial contamination regarding bacteria, fungi, viruses and mycoplasmas were exclusively negative; Lactate Dehydrogenase (LDH) and Malate Dehydrogenase (MDH) polymorphism analysis disproved the existence of cross-contamination from other cell lines; diploid cells making up 85-93% of the population verified the hereditary stability of the fibroblast line. pEGFP-N3, pEYFP-N1 and pDsRed1-N1 were transfected into the Qing Kedan chicken embryonic fibroblasts with transfection efficiencies between 12.6 and 39.7%. To locate their. cellular distribution, transfected cells were observed at 24, 48 and 72 h with confocal microscopy and the fluorescences could be observed throughout the cytoplasm and nuclei of positive cells except in. cryptomere vesicles. This research preserved the precious germplasm resource of Qing Kedan chicken at cell level and hence served as an enlightening reference for those of other poultry in the world.

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