Establishment and Biological Characteristic Research of Yuxizhiwei sheep Fibroblast Cell Bank

文献类型： 外文期刊

第一作者： Wang, Hui

作者： Wang, Hui;Li, Xiangchen;Li, Changli;Zhang, Wenxiu;Guan, Weijun;Ma, Yuehui;Wang, Hui

作者机构：

关键词： Yuxizhiwei sheep;fibroblast cell;biological characteristics;genetic resource conservation

期刊名称：LIFE SCIENCE JOURNAL-ACTA ZHENGZHOU UNIVERSITY OVERSEAS EDITION （ 影响因子：0.165； ）

ISSN： 1097-8135

年卷期： 2012 年 9 卷 4 期

页码：

收录情况： SCI

摘要： An ear marginal fibroblast cell bank was established from the Yuxizhiwei sheep which is an excellent Chinese livestock breed using attachment culture and freezing biotechniques. This bank included 30 ear samples and had stocks of 136 cryogenically preserved vials, each containing 1.7x10(7) cells. Establishment and biological characteristic research results of the cell bank showed that the cells revealed typical fibroblast morphology and grew well in vitro, the growth curve consisted of latent phase, logarithmic growth phase and stationary phase, the cell population doubling time (PDT) was 24 hours, and there was no microbe contamination (bacteria, epiphyte, virus or mycoplasma) in the culture. In addition, lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) zymography indicated that this cell bank was free of cross-contamination. We also determined that the diploid rate of the cell bank was 90.2%similar to 91.4% and measured the expression ratios of three fluorescent protein genes which were between 11.3% and 30.2%. The quality identity of this cell bank thus satisfied the standards of the American Type Culture Collection (ATCC). This study has not only opened up new ways to conserve genetic resources of important and endangered animal breeds in the form of somatic cells, but also provided valuable experimental materials for cell biology, medicine, genomics, post-genomics, embryo engineering and so on. [Wang H, Li XC, Li CL. Establishment and Biological Characteristic Research of Yuxizhiwei sheep Fibroblast Cell Bank. Life Sci J 2012;9(4):3487-3493] (ISSN: 1097-8135). http://www.lifesciencesite.com. 517

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