文献类型: 外文期刊
第一作者: Wang, Hui
作者: Wang, Hui;Li, Xiangchen;Wang, Guangbin;He, Xiaohong;Guan, Weijun;Ma, Yuehui;Wang, Hui
作者机构:
关键词: Garlicin;Mouse myeloma cell line SP2/0;Apoptosis
期刊名称:LIFE SCIENCE JOURNAL-ACTA ZHENGZHOU UNIVERSITY OVERSEAS EDITION ( 影响因子:0.165; )
ISSN: 1097-8135
年卷期: 2012 年 9 卷 4 期
页码:
收录情况: SCI
摘要: In order to investigate the proliferative and apoptotic effects of garlicin on mouse myeloma cell line SP2/0, and to explore the mechanisms of garlicin induced apoptosis, mouse myeloma cell line SP2/0 was used as research material. morphological events and calcium dynamics were observed with phase contrast and confocal microscopy, mitochondrial transmembrane potential, cell cycle progression and apoptotic rates were detected using flow cytometry, and TUNEL staining and agarose gel electrophoresis were performed upon exposure to the drug. Garlicin exerts a strong inhibitory effect on SP2/0 cell line, which is dosage and duration dependent. There occured typical apoptotic morphological alterations at 48h upon exposure. Calcium dynamics were disturbed, cell cycle was arrested at G0/G1 checkpoint, and the ratio of cells in G0/G1 phase was gradually increased while the proportion in S phase was reduced with elevated concentration. Mitochondrial transmembrane potential dropped. The viable-apoptotic and non-viable apoptotic rates were both dose-dependent. Agarose gel electrophoresis revealed that "DNA ladders", a symptom of DNA fragmentation, had taken place. Garlicin could effectively inhibit the proliferation of SP2/0 cell line, influence cell cycle progression and induce apoptosis. [Wang H, Li XC, Wang GB. The proliferative and apoptotic effects of garlicin on mouse myeloma cell line SP2/0. Life Sci J 2012; 9(4):3587-3594] (ISSN: 1097-8135). http://www.lifesciencesite.com. 531
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