Efficiency of in vitro embryo production of yak (Bos grunniens) cultured in different maturation and culture conditions

文献类型： 外文期刊

第一作者： Guo, Xian

作者： Guo, Xian;Ding, Xuezhi;Pei, Jie;Bao, Pengjia;Liang, Chunnian;Chu, Min;Yan, Ping;Guo, Xian;Ding, Xuezhi;Pei, Jie;Bao, Pengjia;Liang, Chunnian;Chu, Min;Yan, Ping

作者机构：

关键词： oocyte;in vitro maturation;in vitro fertilisation;granulosa cell;embryo;yak

期刊名称：JOURNAL OF APPLIED ANIMAL RESEARCH （ 影响因子：1.63； 五年影响因子：1.727 ）

ISSN： 0971-2119

年卷期： 2012 年 40 卷 4 期

页码：

收录情况： SCI

摘要： A study was conducted to evaluate the potential of immature oocytes from the ovaries of yak. The effects of maturation times (23-30 h), hormones (FSH, LH, E-2), sera (fetal calf serum, FCS, or oestrous cow serum, ECS) and culture system (co-culture with granulosa cell, GC, or with bovine oviduct epithelia cells, BOEC, tissue culture medium, TCM, 199 absence co-culture cell, and synthetic oviduct fluid with amino acids, SOFaa) on the in vitro development of in vitro matured and fertilised yak oocytes were examined. Immature oocytes surrounded with compacted cumulus cell were cultured for 23-30 h in TCM 199 supplemented with 10% FCS and hormones. In vitro fertilisation (IVF) was performed with frozen-thawed, caffeine and heparin treated spermatozoa from Datong yak. Oocytes were incubated with 1 x 10(6)/ml spermatozoa for 16-18 h and then cultured in co-culture system with GC or BOEC and/or SOFaa for 7-8 days, respectively. Cleavage and development to blastocysts were recorded on days 2 and 8, respectively, after the start of culture. 27-28 h was superior to other culture times and addition hormones (FSH, LH, E-2) were superior to absence either of them for oocyte maturation. The best maturation of oocyte in TCM 199 with 5.0 mg/L LH, 0.5 mg/L FSH, 1 mg/L E-2. FCS was superior to ECS for development to blastocysts. Co-culture with GC was superior to SOFaa and TCM 199 absence co-culture cell for cleavage, development to blastocysts. The results show that choice of culture conditions has marked effect on the development of in vitro matured and fertilised yak oocytes. The present results indicate that the co-culture with GCs is the most important factor for IVF to development into blastocysts of yak oocytes matured in vitro.

分类号：

  • 相关文献

[1]The CXCL12-CXCR4 signaling promotes oocyte maturation by regulating cumulus expansion in sheep. Zhang, Rui-Na,Pang, Bo,Guo, Song-Chang,Jia, Gong-Xue,Hu, Lin-Yong,Zhao, Xin-Quan,Yang, Qi-En,Xu, Shang-Rong,Wan, Peng-Cheng,Ji, Han-Zhong,Yang, Qi-En. 2018

[2]A protocol for in vitro maturation and fertilization of sheep oocytes. Wang, S,Liu, Y,Holyoak, GR,Evans, RC,Bunch, TD. 1998

[3]Melatonin improves the fertilization capacity and developmental ability of bovine oocytes by regulating cytoplasmic maturation events. Zhao, Xue-Ming,Wang, Na,Hao, Hai-Sheng,Li, Chong-Yang,Zhao, Ya-Han,Wang, Hao-Yu,Du, Wei-Hua,Wang, Dong,Liu, Yan,Pang, Yun-Wei,Zhu, Hua-Bin,Yan, Chang-Liang. 2018

[4]Melatonin enhances the in vitro maturation and developmental potential of bovine oocytes denuded of the cumulus oophorus. Zhao, Xue-Ming,Min, Jiang-Tao,Du, Wei-Hua,Hao, Hai-Sheng,Liu, Yan,Qin, Tong,Wang, Dong,Zhu, Hua-Bin,Min, Jiang-Tao.

[5]In vitro maturation and artificial activation of donkey oocytes. Wu, Kaifeng,Cui, Liang,Zhao, Lixia,Liu, Yiyi,Zhou, Huanmin,Zhao, Gaoping,Tan, Xiuwen.

[6]Predictive value of the area of expanded cumulus mass on development of porcine oocytes matured and fertilized in vitro. Qian, Y,Shi, WQ,Ding, JT,Sha, JH,Fan, BQ.

[7]Effects of N-carbamylglutamate and L-arginine on steroidogenesis and gene expression in bovine granulosa cells. Feng, T.,Schutz, L. F.,Morrell, B. C.,Perego, M. C.,Spicer, L. J.. 2018

[8]Systemic analysis of gene expression profiles in porcine granulosa cells during aging. Li Hui,Guo Shuangshuang,Yu Jianning,Shi Zhendan. 2017

[9]Expression, Regulation, and Functional Characterization of FST Gene in Porcine Granulosa Cells. Zhou, QuanYong,Wan, MingChun,Wei, QiPeng,Song, QiongLi,Xiong, LiGen,Huo, JunHong,Huang, JiangNan.

[10]Transcriptome sequencing analysis of porcine granulosa cells treated with an anti-inhibin antibody. Lei, Mingming,Cai, Liuping,Li, Hui,Chen, Zhen,Shi, Zhendan. 2017

[11]Fas-Associated Death Domain (FADD) Mediated Activation of a Apoptosis Program in Bovine Follicular Granulosa Cells. Yang, Run-Jun,Li, Jun-Ya,Gao, Xue,Zhang, Lu-Pei,Gao, Hui-Jiang,Xu, Shang-Zhong,Yang, Run-Jun,Zhao, Zhi-Hui. 2011

[12]Regulation of FoxO1 transcription factor by nitric oxide and cyclic GMP in cultured rat granulosa cells (Retracted article. See vol 24, pg 281, 2007). Li, Xuebin,Jiang, Yongqing,Wang, Zhengchao,Liu, Gentao,Hutz, Reinhold J.,Liu, Wenbin,Xie, Zhuang,Shi, Fangxiong. 2005

[13]Lipopolysaccharide and heat stress impair the estradiol biosynthesis in granulosa cells via increase of HSP70 and inhibition of smad3 phosphorylation and nuclear translocation. Li, Hui,Cai, Liuping,Shi, Zhendan,Guo, Shuangshuang,Ma, Weiming.

[14]Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion. Hongtuo, F,Chingjiang, W. 2001

[15]Effects of lipopolysaccharide on maturation of bovine oocyte in vitro and its possible mechanisms. Zhao, Shan-Jiang,Pang, Yun-Wei,Zhao, Xue-Ming,Du, Wei-Hua,Hao, Hai-Sheng,Zhu, Hua-Bin. 2017

[16]The Evaluation of Factors Affected on Juvenile in vitro Embryo Transfer in Xinjiang Fine Wool Lamb. Wu, Weiwei,Wang, Feng,Wu, Weiwei,Hanikezi,Tian, Kechuan,Xu, Xinming,Tian, Yuezhen. 2012

[17]Effect of mouse cumulus cells on the in vitro maturation and developmental potential of bovine denuded germinal vesicle oocytes. Zhao, Xue-Ming,Ren, Jing-Jing,Du, Wei-Hua,Hao, Hai-Sheng,Liu, Yan,Qin, Tong,Wang, Dong,Zhu, Hua-Bin.

[18]Effect of vitrification on meiotic maturation, mitochondrial distribution and glutathione synthesis in immature silver fox cumulus oocyte complexes. Cao, Xinyan,Xue, Hailong,Wang, Shiyong,Zhao, Weigang,Du, Zhanyu,Yang, Yifeng,Yue, Zhigang,Cao, Xinyan,Xue, Hailong,Wang, Shiyong,Zhao, Weigang,Du, Zhanyu,Yang, Yifeng,Yue, Zhigang,Li, Jingchun.

[19]Effect of cryotop vitrification method on oocytes at different meiotic stages and embryo development in vitro. Li Xiangchen,Yao Yaxin,Wang Yipeng,Li Heping,Guan Weijun,Ma Yuehui. 2012

[20]Linear Loading Cryoprotectants (CPAs) With Microfluidic Method Reduces Osmotic Damage to Porcine M II-stage Oocytes. Yang Yun,Zhou Xin-Li,Shag Wen-Qi,Yi Xing-Yue,Tao Le-Ren,Dai Jian-Jun,Zhang De-Fu. 2016

作者其他论文 更多>>

微信小程序