Identification of drought-responsive genes by cDNA-amplified fragment length polymorphism in maize

文献类型： 外文期刊

第一作者： Liu, L.

作者： Liu, L.;Hao, Z.;Weng, J.;Li, M.;Zhang, D.;Bai, L.;Li, X.;Zhang, S.;Liu, L.;Wang, L.

作者机构：

关键词： cDNA-amplified fragment length polymorphism;drought tolerance;maize (Zea mays L;);MYB

期刊名称：ANNALS OF APPLIED BIOLOGY （ 影响因子：2.75； 五年影响因子：2.942 ）

ISSN： 0003-4746

年卷期： 2012 年 161 卷 3 期

页码：

收录情况： SCI

摘要： The cDNA-AFLP (amplified fragment length polymorphism) technique was performed to detect transcript-derived fragments related to drought tolerance in maize inbred lines X178 and B73 to identify and understand drought-responsive genes at the transcriptional level. By comparing the sequences of fragments differentially expressed between these lines under water-stressed and well-watered conditions, 72 drought-responsive unique expressed sequence tags (ESTs) were identified with 41 primer combinations targeting PstI/MseI restriction sites. Most of the fragments isolated in these experiments were found to be associated with genes responsive to abiotic or biotic stresses. Subsequently, a putative R1-type MYB transcription factor gene corresponding to ZmMYB-R1 on bin 9.03 was obtained by in silico cloning by homology to EST sequence P36M87-366, which matched one of the AFLPs identified in this study. The 1881 bp sequence encodes a protein of 626 amino acids that contains a single conserved MYB-like DNA-binding domain. Expression of green fluorescence protein fusions showed that ZmMYB-R1 was localised in the cell nucleus. Quantitative real-time polymerase chain reaction revealed that ZmMYB-R1 was induced by drought, high salt, heat, cold and exogenous ABA in maize. The expression of ZmMYB-R1 initially reaches its highest levels in leaves, then is subsequently also detected in stems and roots. This work revealed genes potentially responsible for drought tolerance in maize, and a new gene, ZmMYB-R1, was shown to be associated with water stress.

分类号：

  • 相关文献

[1]Isolation and expression analysis of the soybean GmPic gene. Sha, A. -H.,Zhou, X. -A.,Wu, H.,Fu, X. -M.,Zhang, Q. -L.,Guo, Q. -L.,Chen, Y. -H.,Sha, A. -H.. 2014

[2]Decreased sorbitol synthesis leads to abnormal stamen development and reduced pollen tube growth via an MYB transcription factor, MdMYB39L, in apple (Malus domestica). Meng, Dong,He, Mingyang,Xu, Hongxia,Cheng, Lailiang,He, Mingyang,Bai, Yang,Fei, Zhangjun,Xu, Hongxia,Dandekar, Abhaya M.. 2018

[3]Over-expression of GmMYB39 leads to an inhibition of the isoflavonoid biosynthesis in soybean (Glycine max. L). Liu, Xiaoqing,Xu, Ling,Xu, Zhaolong,Huang, Yihong,He, Xiaolan,Ma, Hongxiang,Yi, Jinxin,Zhang, Dayong,Yuan, Lingling.

[4]Functional Characterization of the Versatile MYB Gene Family Uncovered Their Important Roles in Plant Development and Responses to Drought and Waterlogging in Sesame. Mmadi, Marie Ali,Dossa, Komivi,Wang, Linhai,Zhou, Rong,Wang, Yanyan,Zhang, Xiurong,Mmadi, Marie Ali,Dossa, Komivi,Cisse, Ndiaga,Mmadi, Marie Ali,Dossa, Komivi,Sy, Mame Oureye. 2017

[5]A R2R3-MYB Transcription Factor Regulates the Flavonol Biosynthetic Pathway in a Traditional Chinese Medicinal Plant, Epimedium sagittatum. Huang, Wenjun,Khaidun, A. B. M.,Chen, Jianjun,Lv, Haiyan,Wang, Ying,Zhang, Chanjuan,Yuan, Ling. 2016

[6]A wheat R2R3-MYB gene, TaMYB30-B, improves drought stress tolerance in transgenic Arabidopsis. Zhang, Lichao,Zhao, Guangyao,Xia, Chuan,Jia, Jizeng,Liu, Xu,Kong, Xiuying. 2012

[7]Identification of TT2 gene from floral transcriptome in Fagopyrum tataricum. Zhou, Mei-Liang,Shao, Ji-Rong,Zhou, Mei-Liang,Tang, Yi-Xiong,Wu, Yan-Min,Zhou, Mei-Liang,Zhang, Kai-Xuan,Tang, Yu,Li, Fa-Liang,Yang, Pei-Yan.

[8]Characterization of a Wheat R2R3-MYB Transcription Factor Gene, TaMYB19, Involved in Enhanced Abiotic Stresses in Arabidopsis. Zhang, Lichao,Liu, Guoxiang,Zhao, Guangyao,Xia, Chuan,Jia, Jizeng,Liu, Xu,Kong, Xiuying.

[9]Genome-Wide Identification and Analysis of the MYB Transcription Factor Superfamily in Solanum lycopersicum. Li, Zhenjun,Peng, Rihe,Tian, Yongsheng,Han, Hongjuan,Xu, Jing,Yao, Quanhong.

[10]Isolation and functional characterization of a R2R3-MYB regulator of the anthocyanin biosynthetic pathway from Epimedium sagittatum. Huang, Wenjun,Khaldun, A. B. M.,Lv, Haiyan,Du, Liuwen,Wang, Ying,Du, Liuwen,Zhang, Chanjuan.

[11]The wheat MYB transcription factor TaMYB18 regulates leaf rolling in rice. Zhang, Lichao,Dong, Chunhao,Zhang, Qiang,Zhao, Guangyao,Li, Fu,Xia, Chuan,Zhang, Lina,Han, Longzhi,Jia, Jizeng,Liu, Xu,Kong, Xiuying,Wu, Jinxia.

[12]Colored light-quality selective plastic films affect anthocyanin content, enzyme activities, and the expression of flavonoid genes in strawberry (Fragaria x ananassa) fruit. Miao, Lixiang,Zhang, Yuchao,Yang, Xiaofang,Xiao, Jinping,Zhang, Huiqin,Zhang, Zuofa,Wang, Yuezhi,Jiang, Guihua.

[13]Constructing a high-density linkage map for Gossypium hirsutum x Gossypium barbadense and identifying QTLs for lint percentage. Yuzhen Shi,Wentan Li,Aiguo Li,Ruihua Ge,Baocai Zhang,Junzhi Li,Guangping Liu,Junwen Li,Aiying Liu,Haihong Shang,Juwu Gong,Wankui Gong,Zemao Yang,Feiyü Tang,Zhi Liu,Weiping Zhu,Jianxiong Jiang,Xiaonan Yu,Tao Wang,Wei Wang,Tingting Chen,Kunbo Wang,Zhengsheng Zhang,Youlu Yuan. 2015

[14]Deep Roots are Pivotal for Regulating Post-Anthesis Leaf Senescence in Wheat (Triticum aestivum L.). Kong, L.,Si, J.,Sun, M.,Feng, B.,Zhang, B.,Li, S.,Wang, Z.,Wang, F.,Wang, F.. 2013

[15]Rice transgene flow: its patterns, model and risk management. Jia, Shirong,Pei, Xinwu,Wang, Zhixing,Yuan, Qianhua,Wang, Feng,Hu, Ning,Yao, Kemin.

[16]Activation tagging, an efficient tool for functional analysis of the rice genome. Wan, Shuyan,Wu, Jinxia,Zhang, Zhiguo,Sun, Xuehui,Lv, Yaci,Gao, Ci,Ning, Yingda,Ma, Jun,Guo, Yupeng,Zhang, Qian,Zheng, Xia,Lu, Tiegang,Lv, Yaci,Gao, Ci,Ning, Yingda,Zhang, Caiying,Ma, Zhiying.

[17]Mutation of the rice ASL2 gene encoding plastid ribosomal protein L21 causes chloroplast developmental defects and seedling death. Lin, D.,Jiang, Q.,Zheng, K.,Chen, S.,Zhou, H.,Gong, X.,Dong, Y.,Chen, S.,Zhou, H.,Teng, S.,Xu, J..

[18]Identification and Comparative Analysis of Premature Senescence Leaf Mutants in Rice (Oryza sativa L.). He, Yan,Li, Liangjian,Zhang, Zhihong,Wu, Jian-Li. 2018

[19]OsMOGS is required for N-glycan formation and auxin-mediated root development in rice ( Oryza sativa L.). Wang, SuiKang,Xu, YanXia,Zhang, SaiNa,Jiang, De An,Qi, YanHua,Li, ZhiLan,Lim, Jae-Min,Lim, Jae-Min,Lee, Kyun Oh,Lee, Kyun Oh,Li, ChuanYou,Qian, Qian. 2014

[20]Wheat genotypes differing in aluminum tolerance differ in their growth response to CO2 enrichment in acid soils. Tian, Qiuying,Zhang, Xinxin,Gao, Yan,Bai, Wenming,Zhang, Wen-Hao,Ge, Feng,Ge, Feng,Zhang, Wen-Hao,Ma, Yibing. 2013

作者其他论文 更多>>

微信小程序