Isolation and Purification of Polysaccharides with Anti-tumor Activity from Pholiota adiposa (Batsch) P. Kumm. (Higher Basidiomycetes)
文献类型: 外文期刊
第一作者: Ng, Tzi Bun
作者: Ng, Tzi Bun;Hu, Qingxiu;Wang, Hexiang;Hu, Qingxiu;Wang, Hexiang;Hu, Qingxiu
作者机构:
关键词: medicinal mushrooms;Pholiota adiposa;polysaccharide;purification;anti-tumor activity;splenic lymphocyte proliferation
期刊名称:INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS ( 影响因子:1.921; 五年影响因子:1.879 )
ISSN: 1521-9437
年卷期: 2012 年 14 卷 3 期
页码:
收录情况: SCI
摘要: Pholiota adiposa is a mushroom with excellent medicinal and nutritional properties. After culture in fermentation medium, Ph. adiposa mycelia were filtered, lyophilized, and powdered. A crude polysaccharide (PAP) of Ph. adiposa was prepared from the mycelial powder with hot water, centrifuged, and the resulting supernatant lyophilized. PAP was fractionated by 30%, 60%, and 80% ethanol precipitation steps to yield PAP30, PAP60, and PAP80. Subsequently PAP30-1 and PAP30-2, PAP60-1 and PAP60-2, and PAP80-1 and PAP80-2 were isolated from PAP30, PAP60, and PAP80, respectively, by ion-exchange chromatography on a DEAE-Sepharose column. Polysaccharide content increased from 43.8% in PAP to 50.54%similar to 73.19% in PAP30-1 similar to PAP80-2. The protein content was 4.92% at minimum in these polysaccharide products. In order to identify the chemical composition, the six polysaccharides (PAP30-1, PAP30-2, PAP60-1, PAP60-2, PAP80-1, and PAP80-2) were further purified by gel filtration on Sephacryl S-100-500. Finally, three water-soluble polysaccharides (PAP30-2a, PAP60-2b, and PAP80-2a) were obtained. HPLC analysis revealed that PAP30-2a, PAP60-2b, and PAP80-2a exhibited a molecular weight of 6.6 x 10(5) Da, 8.4 x 10(3) Da, and 3.5 x 10(3) Da, respectively. The glucose content in PAP80-2a, PAP60-2b, and PAP30-2a was 57.8%, 72.7%, and 68.9%, respectively. PAP30-2a, PAP60-2b, and PAP80-2a demonstrated significant differences in anti-tumor activity in mice. PAP80-2a is the optimal bioactive constituent with anti-tumor and T-lymphocyte proliferation stimulating effects.
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