DNA methylation analysis of exon-1 of the ovine HOXC-8 gene in Mongolian sheep using bisulphite sequencing
文献类型: 外文期刊
第一作者: Zhang, Liling
作者: Zhang, Liling;Zhao, Jing;Chen, Qi;Ma, Yuehui
作者机构:
关键词: bisulphite sequencing PCR;DNA methylation;multi-vertebrae phenotype
期刊名称:JOURNAL OF APPLIED ANIMAL RESEARCH ( 影响因子:1.63; 五年影响因子:1.727 )
ISSN: 0971-2119
年卷期: 2012 年 40 卷 3 期
页码:
收录情况: SCI
摘要: The multi-vertebrae phenotype of the Mongolian sheep is not explained by Mendel's law. To test the hypothesis that the multi-vertebrae phenotype is related to HOXC-8 mutation or genomic imprinting, the sequences of exon-1 and exon-2 of the HOXC-8 gene of normal and multi-vertebrae Mongolian sheep were analysed. Bisulphite sequencing Polymerase Chain Reaction (PCR) was employed to compare the methylation status of cytosine-guanine (CpG) sites in HOXC-8 exon-1 region from normal and multi-vertebrae Mongolian sheep. No mutations were observed in HOXC-8 exon-1 or exon-2 of normal and multi-vertebrae Mongolian sheep. However, the level of DNA methylation in exon-1 ofthe HOXC-8 gene was much lower in the 13-vertebrae sheep than in the 14-vertebrae sheep. In the CpG island (293 bp), there were six, three and six methylated CpGs in the normal sheep samples and 23, 20 and 21 in the multi-vertebrae sheep samples, respectively. The ratio of methylated to non-methylated CpG dinucleotides in normal and multi-vertebrae sheep were 18.50 +/- 0.064 and 79.03 +/- 0.056% (P<0.01, by a t-test), respectively.
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