Determination of Digestive Enzyme Activity in the Digesta from the Small Intestinal of Growing Pigs and Development of in vitro Evaluation System for Feed Bioavailability Using Artificial Small Intestinal Juice

文献类型： 外文期刊

第一作者： Fang, Luoyun

作者： Fang, Luoyun;Wang, Junjun;Li, Defa;Zhang, Hongfu;Zhao, Feng

作者机构：

关键词： Digestive enzyme activity;growing pig;in vitro;feed evaluation;intestinal fluid;China

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2012 年 11 卷 11 期

页码：

收录情况： SCI

摘要： The objective of this study was to determine enzyme activity in the digesta from the small intestine of growing pigs and develope an in vitro evaluation system for feed bioavailability using artificial small intestinal juice. Ten pigs (22.77 +/- 0.89 kg and fitted with a simple T-cannula at the jejunum) were used in a doubly 5x5 Latin square design. In each period, pigs were offered one of five diets differing in nutrient level for 14 days. The Standard diet (ST) contained 3400 kcal kg(-1) of Digestible Energy value (DE) and 17% Crude Protein (CP). The DE for the other four diets was 3600, 3200, 3600 and 3200 kcal kg(-1), respectively while the CF level was 21, 13, 13 and 21%, respectively. The small intestinal digesta was collected for determining digestive enzyme activity. Meanwhile, feces were collected for determining in vivo feed digestibility. The results showed that the range of amylase, trypsin, chymotrypsin and lipase activities in the intestinal fluid of growing pigs was 15.52-251.43, 21.24-67.39, 3.45-19.17 and 0.02-3.59 U mL(-1), respectively. To establish an in vitro evaluation system for feed bioavailability, Artificial Small Intestinal Juice (ASIJ) was prepared with mixed enzyme reagents based on the mean activities of amylase, trypsin, chymotrypsin and lipase in the digesta used to evaluate the five diets by three-stage enzymatic incubation. By comparing the DE of the five feedstuffs from the in vivo method and the digestibility of the feedstuffs from the in vitro ASIJ analysis, mathematical models for predicting in vivo DE of In Vitro Dry Matter (IVDM), Organic Matter (IVOM) and DE (IVDE) was established (DE = 0.1076xIVDM+0.3741, R-2 = 0.34; DE = 0.1276xIVOM+1.6486, R-2 = 0.31; DE = 0.4625xIVDE+7.2065, R-2 = 0.71). There were no significant differences between the in vitro evaluation results and the developed in vitro method. Therefore, the system in this study based on ASIJ is a convinent and reasonably accurate method for in vitro evaluation of feed bioavailability.

分类号：

  • 相关文献

[1]Effect of copper, ferrous iron and zinc from different sources on growth performance and fecal copper, ferrous iron and zinc balance in growing pigs. Ying, Peng,Deng, Yuelin,Chen, Feng,Deng, Dun,Deng, Yuelin,Chen, Feng,Fang, Jun,Mi, Many,Wang, Mi,Min, Gong,Min, Gong,Liu, Gang,Wang, Yongfei,Yin, Ronghua,Fan, M. Z.. 2010

[2]Effects of bioflocs on water quality, and survival, growth and digestive enzyme activities of Litopenaeus vannamei (Boone) in zero-water exchange culture tanks. Xu, Wu Jie,Pan, Lu Qing,Sun, Xiao Hua,Huang, Jie. 2013

[3]Effect of water temperature on digestive enzyme activity and gut mass in sea cucumber Apostichopus japonicus (Selenka), with special reference to aestivation. Gao Fei,Yang Hongsheng,Xu Qiang,Wang Fangyu,Liu Guangbin,Gao Fei. 2009

[4]Effects of dietary medicinal herbs and Bacillus on survival, growth, body composition, and digestive enzyme activity of the white shrimp Litopenaeus vannamei. Yu, Ming-Chao,Li, Zhuo-Jia,Lin, Hei-Zhao,Wen, Guo-Liang,Ma, Shen.

[5]Effects of dietary protein source on the digestive enzyme activities and electrolyte composition in the small intestinal fluid of chickens. Ren, L. Q.,Zhao, F.,Zhang, J. Z.,Zhang, H. F.,Tan, H. Z.,Zhao, J. T.. 2012

[6]Carbon adaptation influence the antagonistic ability of Pseudomonas aeruginosa against Fusarium oxysporum f. sp melonis. Wang, Yanli,Sun, Guochang,Li, Bin,Tang, Qiaomei,Chen, Xiaoling,Xie, Guanlin,Li, Hongye,Yu, Rongrong,Wu, Zhiyi. 2011

[7]In vitro and in vivo antioxidative and radioprotective capacities of polysaccharide isolated from Mesona Blumes gum. Feng, Tao,Sang, Min,Zhuang, Haining,Xu, Zhimin,Zhuang, Haining,Xu, Zhimin. 2017

[8]In vitro organogenesis and plant regeneration from leaves of Actinidia eriantha Benth. cv White (kiwifruit). Wu, Y. J.,Xie, M.,Long, Q. J.. 2011

[9]The preparation of neem oil microemulsion (Azadirachta indica) and the comparison of acaricidal time between neem oil microemulsion and other formulations in vitro. Xu, Jiao,Fan, Qiao-Jia,Yin, Zhong-Qiong,Du, Yong-Hua,Jia, Ren-Yong,Wang, Kai-Yu,Lv, Cheng,Ye, Gang,Geng, Yi,Su, Gang,Zhao, Ling,Hu, Ting-Xiu,Shi, Fei,Zhang, Li,Wu, Chang-Long,Tao, Cui,Zhang, Ya-Xue,Shi, Dong-Xia,Li, Xu-Ting,Yin, Zhong-Qiong,Jia, Ren-Yong,Yin, Zhong-Qiong.

[10]Optimization of droplet-vitrification protocol for carnation genotypes and ultrastructural studies on shoot tips during cryopreservation. Zhang, Jin-Mei,Zhang, Xiao-Ning,Xin, Xia,Yin, Guang-Kun,He, Juan-Juan,Chen, Xiao-Ling,Zhang, Xiao-Ning,Xu, You-Ming,Zhang, Xiao-Ning.

[11]The Antiviral Effects of Harmine Against BoHV-1 Infection In Vitro. Jiang, Xinyi,Zou, Jie,Zhuang, Yuncheng,Zhu, Liqian,Zhu, Guoqiang,Yuan, Weifeng.

[12]Infection of small ruminants and their red blood cells with Theileria annulata schizonts. Yin, Hong. 2014

[13]Porcine Mx1 fused to HIV Tat protein transduction domain (PTD) inhibits classical swine fever virus infection in vitro and in vivo. Jing, Jiao,Shi, Baojun,Xu, Qianqian,Zhou, Bin,Chen, Puyan,Li, Wenliang,Liu, Ke,Ma, Zhiyong. 2015

[14]Cultivation and Biological Characterization of Chicken Primordial Germ Cells. Ji, Meng,Guan, Wei-jun,Gao, Yu-hua,Bai, Chun-yu,Ma, Yue-hui,Li, Xiang-chen,Li, Lu. 2016

[15]Synthesis and In Vitro Anticancer Activity of Novel 2-((3-thioureido)carbonyl)phenyl Acetate Derivatives. Xiong, Lin,Gao, Ya-Qin,Niu, Chu-E,Wang, Hong-Bo,Li, Wei-Hong,Xiong, Lin,Gao, Ya-Qin,Niu, Chu-E,Wang, Hong-Bo,Li, Wei-Hong. 2014

[16]Acaricidal activity of petroleum ether extract of neem (Azadirachta indica) oil and its four fractions separated by column chromatography against Sarcoptes scabiei var. cuniculi larvae in vitro. Deng, Yunxia,Shi, Dongxia,Yin, Zhongqiong,Jia, Renyong,Xu, Jiao,Song, Xu,Lv, Cheng,Fan, Qiaojia,Liang, Xiaoxia,Shi, Fei,Ye, Gang,Zhang, Wei,Guo, Jianhong,Yin, Zhongqiong,Jia, Renyong. 2012

[17]Disintegration of starch crystal structure by steam flaking may be responsible for the improvement of in vitro ruminal fermentation of steam flaked sorghum grains. Ding, J.,Wan, R.,Ren, L. P.,Meng, Q. X.,Zhang, L.. 2007

[18]Comparison of the structural characterization and biological activity of acidic polysaccharides from Cordyceps militaris cultured with different media. Wu, Fengyao,Yan, Hui,Ma, Xiaoning,Jia, Junqiang,Zhang, Guozheng,Guo, Xijie,Gui, Zhongzheng,Jia, Junqiang,Zhang, Guozheng,Guo, Xijie,Gui, Zhongzheng. 2012

[19]Characterization of GTMAC-Graft-Chitosan as a Bactericidal Agent for Nitric Oxide Release. Sun, Yan,Yang, Peng,Liu, Yong,Xu, Yaoxing,Li, Yanli,Xu, Shaochun. 2011

[20]Angiotensin I Converting Enzyme (ACE) inhibitory activity and antihypertensive effects of grass carp peptides. Wang Shen,Lin Li-mei,Wu Yong-ning,Fang Min,Yu Ya-qin,Zhou Jia,Gong Zhi-yong,Wu Yong-ning. 2014

作者其他论文 更多>>

微信小程序