A Fragment of Cadherin-Like Protein Enhances Bacillus thuringiensis Cry1B and Cry1C Toxicity to Spodoptera exigua (Lepidoptera: Noctuidae)

文献类型： 外文期刊

第一作者： Lu Qiong

作者： Lu Qiong;Zhang Yong-jun;Cao Guang-chun;Zhang Li-li;Liang Ge-mei;Lu Yan-hui;Wu Kong-ming;Guo Yu-yuan;Lu Qiong;Zhang Li-li;Gao Xi-wu

作者机构：

关键词： Bacillus thuringiensis;cadherin-like;Spodoptera exigua;enhancement toxicity

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2012 年 11 卷 4 期

页码：

收录情况： SCI

摘要： Cry toxins produced by Bacillus thuringiensis (Bt) are effective biological insecticides against certain insect species. In this study, bioassay results indicated that Cry1B and Cry1C were toxic to Spodoptera exigua. We also identified a cadherin-like gene in S. exigua that could enhance the toxicity of Cry1B and Cry1C. The cadherin-like gene identified from the larvae midgut tissue was cloned by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The full-length cDNA of the gene consisted of 5 220 bp encoding I 740 amino acid with a predicted molecular mass of 196 kD. BLAST search analysis showed that the predicted amino acid sequence had a high sequence identity to the published sequences of cadherin-like proteins from other Lepidoptera insects. Spatial expression of the cadherin-like gene detected by qRT-PCR analysis revealed that the cadherin-like gene was mainly present in the gut of 4th instar larvae and during different life stages. The results suggested that the commercial development of this synergist has the potential to enhance Cry1B and Cry1C toxicity against Lepidoptera insects.

分类号：

  • 相关文献

[1]Temporal allocation of metabolic tolerance to transgenic Bt cotton in beet armyworm, Spodoptera exigua (Hubner). Wu Gang,Wan FangHao,Wu Gang. 2011

[2]Bacillus thuringiensis insecticidal crystal proteins affect lifespan and reproductive performance of helicoverpa armigera and spodoptera exigua adults. YING ZHANG,YAN MA,PIN-JUN WAN,LI-LI MU,GUO-QING LI.

[3]Brusatol isolated from Brucea javanica (L.) Merr. induces apoptotic death of insect cell lines. Feng, Xuehuan,Ma, Dejun,Yang, Jingjing,Jiang, Hongyun,Zhang, Yanning,He, Weizhi. 2013

[4]Narrow tuning of an odorant receptor to plant volatiles in Spodoptera exigua (Hubner). Liu, Yang,Guo, Mengbo,Cao, Depan,Wang, Guirong,Liu, Chengcheng,Guo, Mengbo,Dong, Shuanglin. 2014

[5]Molecular characterization of a ryanodine receptor gene from Spodoptera exigua and its upregulation by chlorantraniliprole. Qiu, Guisheng,Ma, Chunsen,Sun, Lina,Qiu, Guisheng,Cui, Li,Ma, Chunsen,Yuan, Huizhu,Cui, Li,Ma, Chunsen,Yuan, Huizhu.

[6]Molecular characterization and expression of vitellogenin gene from Spodoptera exigua exposed to cadmium stress. Zhao, Jing,Sun, Yang,Xiao, Liubin,Tan, Yongan,Bai, Lixin.

[7]Establishment and characterization of three embryonic cell lines of beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae). Su, Rui,Zheng, Gui-Ling,Wan, Fang-Hao,Li, Chang-You,Wan, Fang-Hao.

[8]Synthesis, insecticidal activity and inhibition on topoisomerase I of 20(S)-t-Boc-amino acid derivatives of camptothecin. Li Zhe,Zhang Lan,Zhang Yanning,Mao Liangang,Jiang Hongyun.

[9]Substitutions in Spodoptera exigua topoisomerase I modulate its relaxation activity and camptothecin sensitivity. Zhang, Lan,Zhang, Yanning,Mao, Liangang,Jiang, Hongyun.

[10]Genetic variation, phylogeographic structure of Spodoptera exigua in the welsh onion-producing areas of North China. Wang, X. Y.,Zhong, T.,Xu, G. Q.,Zhou, L. H..

[11]Vitellogenin and vitellogenin receptor gene expression profiles in Spodoptera exigua are related to host plant suitability. Zhao, Jing,Sun, Yang,Xiao, Liubin,Tan, Yongan,Jiang, Yiping,Bai, Lixin,Zhao, Jing. 2018

[12]Effect of host plants on the infectivity of nucleopolyhedrovirus to Spodoptera exigua larvae. Wan, N. -F.,Li, B.,Wan, N. -F.,Jiang, J. -X..

[13]Isolation and Expression Analysis of CYP9A11 and Cytochrome P450 Reductase Gene in the Beet Armyworm (Lepidoptera: Noctuidae). Feng, Xiaoyun,Tang, Tao,Qiu, Lihong,Zhao, Chunqing,Tang, Tao. 2015

[14]Enhancement of attraction to sex pheromones of Spodoptera exigua by volatile compounds produced by host plants. Deng, JY,Wei, HY,Huang, YP,Du, JW. 2004

[15]Cloning of the heat shock protein 90 and 70 genes from the beet armyworm, Spodoptera exigua, and expression characteristics in relation to thermal stress and development. Zhai, Huifang,Wang, Ling,Luo, Lizhi,Zhang, Lei,Sappington, Thomas W.. 2012

[16]Expression of Cry1Ac in transgenic Bt soybean lines and their efficiency in controlling lepidopteran pests. Li, Yunhe,Li, Xiangju,Romeis, Joerg,Wu, Kongming,Romeis, Joerg. 2013

[17]Genetic effects on flight capacity in the beet armyworm, Spodoptera exigua (Lep., Noctuidae). Zhai, B. P.,Zhang, X. X.,Han, L. Z.,Gu, H. N.. 2009

[18]Identification and functional characterization of sex pheromone receptors in beet armyworm Spodoptera exigua (Hubner). Liu, Yang,Wang, Guirong,Liu, Chengcheng,Dong, Shuanglin,Walker, William B.. 2013

[19]Relationship of flight and reproduction in beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae), a migrant lacking the oogenesis-flight syndrome. Jiang, Xing Fu,Luo, Li Zhi,Sappington, Thomas W.. 2010

[20]Response of multiple generations of beet armyworm, Spodoptera exigua (Hubner), feeding on transgenic Bt cotton. Guo, J. -Y.,Wan, F. -H.,Harris, M. K.. 2009

作者其他论文 更多>>

微信小程序