Mapping QTL controlling maize deep-seeding tolerance-related traits and confirmation of a major QTL for mesocotyl length

文献类型： 外文期刊

第一作者： Wang, Guoying

作者： Wang, Guoying;Zhang, Hongwei;Ma, Pan;Zhao, Zhengnan;Zhao, Guangwu;Tian, Baohua;Wang, Jianhua;Zhang, Hongwei;Ma, Pan;Zhao, Zhengnan;Zhao, Guangwu;Tian, Baohua;Wang, Jianhua

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2012 年 124 卷 1 期

页码：

收录情况： SCI

摘要： Deep-seeding tolerant seeds can emerge from deep soil where the moisture is suitable for seed germination. Breeding deep-seeding tolerant cultivars is becoming increasingly important in arid and semi-arid regions. To dissect the quantitative trait loci (QTL) controlling deep-seeding tolerance traits, we selected a tolerant maize inbred line 3681-4 and crossed it with the elite inbred line-X178 to generate an F-2 population and the derivative F-2:3 families. A molecular linkage map composed of 179 molecular markers was constructed, and 25 QTL were detected including 10 QTL for sowing at 10 cm depth and 15 QTL for sowing at 20 cm depth. The QTL analysis results confirmed that deep-seeding tolerance was mainly caused by mesocotyl elongation and also revealed considerable overlap among QTL for different traits. To confirm a major QTL on chromosome 10 for mesocotyl length measured at 20 cm depth, we selected and self-pollinated a BC3F2 plant that was heterozygous at the markers around the target QTL and homozygous at other QTL to generate a BC3F3 population. We found that this QTL explained more phenotypic variance in the BC3F3 population than that in the F-2 population, which laid the foundation for fine mapping and NIL (near-isogenic line) construction.

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