Culturomics and Amplicon-Based Metagenomic Insights into the Bacteria of Soils with High Yield of Oryza sativa L. subsp. Japonica

文献类型： 外文期刊

第一作者： Zhang, Li

作者： Zhang, Li;Li, Yan;Cao, Qingmei;Zhuang, Yan;Ruan, Zhiyong;Cao, Qingmei;Zhuang, Yan;Ruan, Zhiyong;Ruan, Wenzhong;Guo, Yapeng

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关键词： culturomics; 16S rRNA genes; super rice; rhizosphere; non-rhizosphere; bacteria; Oryza sativa L. subsp. Japonica

期刊名称：AGRONOMY-BASEL （ 影响因子：3.7； 五年影响因子：4.0 ）

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年卷期： 2023 年 13 卷 12 期

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收录情况： SCI

摘要： The bacterial community in the paddy field agroecosystem has a pivotal role in the growth adaptability strategy of rice. Here, we studied the bacterial community structure composition of rhizosphere and non-rhizosphere soil samples from super rice in high-yield (920.99 kg/mu) and low-yield (785.30 kg/mu) fields of Japonica Chu 54 using both culturomics and amplicon-based metagenomics approaches. Using amplicon sequencing, a total of 54 phyla and 1167 genera of high-yield field bacteria were detected, while the low-yield field bacteria were distributed in 49 phyla and 865 genera. In addition, compared with low-yielding fields, there were significant differences in the composition and abundance of the same members in high-yielding fields. The node microorganisms in high-yield and low-yield fields were Anaeromyxobacterium and HSB_OF53-F07, respectively. Culturomics analysis unveiled a diverse array of bacterial taxa, encompassing four phyla, 113 genera, and 331 species, including 33 new undescribed lineages. The culturomics and high-throughput sequencing results indicate a widely adapted and highly abundant group of Exiguobacterium, which has broad prospects for application due to its extensive survival characteristics and plant growth-promoting functions. In summary, we analyze the bacterial community structure composition of rhizosphere and non-rhizosphere soil samples from super rice in high-yield and low-yield fields of Japonica Chu 54 using culturomics and amplicon sequencing techniques to better develop positive promotion strategies that adapt to its unique ecological environment.

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