Characterization of acid phosphatase from the tick Haemaphysalis longicornis

文献类型： 外文期刊

第一作者： Zhang, Ping

作者： Zhang, Ping;Tian, Zhancheng;Liu, Guangyuan;Xie, Junren;Luo, Jin;Zhang, Liyan;Shen, Hui

作者机构：

关键词： Haemaphysalis longicornis;Acid phosphatase;Theileria sergenti;Characterization;Anti-tick vaccine

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

ISSN： 0304-4017

年卷期： 2011 年 182 卷 2-4 期

页码：

收录情况： SCI

摘要： The full-length cDNA encoding acid phosphatase (HL-3) from Haemaphysalis longicornis was obtained by 5' rapid amplification of cDNA ends (RACE). The cDNA contained a 1137 bp open reading frame (ORF) coding for 356 amino acids with a predicted theoretical iso-electric point (pl) of 6.35 and molecular weight of 41.0 kDa. The recombinant protein was expressed in Escherichia coli. The enzyme could hydrolyze para-nitrophenyl phosphate (pNPP) substrate at an optimum pH of 5.0. Real-time RT-PCR analysis showed that the HL-3 transcripts were expressed in various stages of unfed ticks and were significantly induced by blood feeding. Furthermore, the expression of HL-3 in midguts was significantly higher than in other tested tissues of partially fed adult ticks. The transcripts of the HL-3 mRNA in lipopolysaccharide (LPS)-injected ticks were 1.75 times of the PBS-injected control; Theileria sergenti infected larvae expressed 3.86 more times than that of uninfected ones. Western blot analysis showed that rabbit antiserum against the recombinant rHL-3 could recognize a native protein of approximately 41.0 kDa in the lysates from different stages of ticks. Vaccination of rabbits with the rHL-3 conferred partial protective immunity against ticks, resulting in 28% mortality and 10.6% reduction in engorgement weight of adult ticks, respectively. These results suggested that the HL-3 was involved in tick innate immunity and could be used as a potential candidate antigen for the development of anti-tick vaccines. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.

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