A pangenomic study of Bacillus thuringiensis

文献类型： 外文期刊

第一作者： Shu, Changlong

作者： Shu, Changlong;Zhang, Jie;Fang, Yongjun;Yu, Jun;Li, Zhaolong;Liu, Jiucheng;Wang, Xumin;Zhang, Xiaowei;Yu, Xiaoguang;Zhao, Duojun;Liu, Guiming;Hu, Songnian;Yu, Jun;Li, Zhaolong;Yu, Jun;Fang, Yongjun;Liu, Jiucheng;Zhang, Xiaowei;Yu, Xiaoguang;Zhao, Duojun;Liu, Guiming;Hu, Songnian;Al-Mssallem, Ibrahim;Yu, Jun

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关键词： Bacillus thuringiensis (B. thuringiensis);Pseudo-chromosome;Pangenome

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：4.275； 五年影响因子：5.223 ）

ISSN： 1673-8527

年卷期： 2011 年 38 卷 12 期

页码：

收录情况： SCI

摘要： Bacillus thuringiensis (B. thuringiensis) is a soil-dwelling Gram-positive bacterium and its plasmid-encoded toxins (Cry) are commonly used as biological alternatives to pesticides. In a pangenomic study, we sequenced seven B. thuringiensis isolates in both high coverage and base-quality using the next-generation sequencing platform. The B. thuringiensis pangenome was extrapolated to have 4196 core genes and an asymptotic value of 558 unique genes when a new genome is added. Compared to the pangenomes of its closely related species of the same genus, B. thuringiensis pangenome shows an open characteristic, similar to B. cereus but not to B. anthracis; the latter has a closed pangenome. We also found extensive divergence among the seven B. thuringiensis genome assemblies, which harbor ample repeats and single nucleotide polymorphisms (SNPs). The identities among orthologous genes are greater than 84.5% and the hotspots for the genome variations were discovered in genomic regions of 2.3-2.8 Mb and 5.0-5.6 Mb. We concluded that high-coverage sequence assemblies from multiple strains, before all the gaps are closed, are very useful for pangenomic studies.

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