文献类型: 外文期刊
第一作者: Cai, Hongying
作者: Cai, Hongying;Shi, Pengjun;Bai, Yingguo;Huang, Huoqing;Yuan, Tiezheng;Yang, Peilong;Luo, Huiying;Meng, Kun;Yao, Bin
作者机构:
关键词: Penicillium pinophilum C1;Xylanase;Thermoacidophilic;Pichia pastoris;Malting and brewing
期刊名称:PROCESS BIOCHEMISTRY ( 影响因子:3.757; 五年影响因子:3.665 )
ISSN: 1359-5113
年卷期: 2011 年 46 卷 12 期
页码:
收录情况: SCI
摘要: A novel endo-beta-1,4-xylanase gene (xyn10C1) was cloned from Penicillium pinophilum C1 and overexpressed in Pichia pastoris. The 1071-bp full-length gene encodes a 356-residue polypeptide containing the catalytic domain of glycoside hydrolase 10. Deduced XYN10C1 shares highest amino acid sequence identity of 49.3% with a putative xylanase from Glomeella graminicola M1.001. Purified recombinant XYN10C1 showed maximal activity at pH 4.0-5.5 and 75 degrees C, exhibited good adaptability to broad acidic pH and temperature ranges (>69.0% activity at pH 2.5-6.5; and >91.0% activity at 70-80 degrees C and 22.2% even at 90 degrees C), and was highly stable at pH 2.0-7.0 for 1 h at 37 degrees C. The specific activity, K-m and V-max values for birchwood xylan and soluble wheat arabinoxylan were 100.7 and 137.4 U/mg, 4.3 and 6.9 mg/ml, and 195.4 and 209.3 mu mol/min/mg, respectively. The enzyme was strongly resistant to most metal ions and proteases (pepsin and trypsin). Under simulated mashing conditions, addition of XYN10C1 (80 U) to the brewery mash (12.5 g in 50 ml system) significantly increased the filtration rate by 26.7% and reduced the viscosity by 9.8%, respectively. All these favorable enzymatic properties make XYN10C1 attractive for potential applications in the food and animal feed industries. (C) 2011 Elsevier Ltd. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Alleviating Clostridium perfringens-Induced Intestinal Lesions in Chickens Using the Xylanase CbXyn10C and Its Binary Cocktail with a Protease
作者:Zhang, Wenjing;Hao, Zhenzhen;Yang, Daoxin;Ji, Wangli;Guo, Kairui;Sun, Xianhua;Wang, Shuai;Yang, Shuyan;Ma, Jianshuang;Luo, Huiying;Yao, Bin;Wang, Yuan;Huang, Huoqing;Su, Xiaoyun;Wang, Tong;Zhang, Meiling
关键词:necrotic enteritis; Clostridium perfringens; xylanase; protease; broiler chickens; gut microbiota modulation
-
p-Hydroxycinnamic Acids: Advancements in Synthetic Biology, Emerging Regulatory Targets in Gut Microbiota Interactions, and Implications for Animal Health
作者:Ma, Chunlai;Liang, Ziqi;Wang, Yuan;Luo, Huiying;Yao, Bin;Tu, Tao;Ma, Chunlai;Liang, Ziqi;Yang, Xiaojun
关键词:acids; bioactivity; animal health; gut microbiota; biosynthesis
-
Fructose metabolism in Entner-Doudoroff pathway-deficient Cupriavidus necator H16 depends on the Calvin shunt
作者:Ding, Lijuan;Guo, Gang;Cui, Lin;Wang, Yuheng;Liu, Xu;Luo, Huiying;Huang, Huoqing;Su, Xiaoyun;Bai, Yingguo;Zhang, Jie;Tu, Tao;Qin, Xing;Wang, Yuan;Wang, Yaru;Yao, Bin;Wang, Xiaolu;Dronsella, Beau;Xue, Xianli
关键词:Cupriavidus necator; Entner-Doudoroff pathway; Calvin shunt; Phosphoglycolate salvage pathway
-
Metabolic Engineering of Escherichia coli for Efficient
l -Isoleucine Production based on the Citramalate Pathway作者:Zhang, Qiquan;Wang, Yuheng;Wang, Xiaolu;Bai, Yingguo;Wang, Yaru;Wang, Yuan;Tu, Tao;Qin, Xing;Su, Xiaoyun;Yao, Bin;Luo, Huiying;Liu, Xu;Huang, Huoqing;Zhang, Jie
关键词:Escherichia coli; l-isoleucine; metabolic engineering; citramalate pathway; threonine-independent pathway
-
Identification of Aspartate Aminotransferase FocAST2 as a Novel Target of Albendazole in Fusarium oxysporum f. sp. cubense TR4
作者:Liu, Yushan;Liu, Siwen;Peng, Cheng;Huang, Huoqing;Zhang, Wei;Huo, Yile;Yi, Ganjun;Li, Chunyu;Zeng, Weiqing
关键词:banana;
Foc TR4; albendazole; target; aspartate transaminase -
Genome-Wide Profiling of bZIP Transcription Factors and FocbZIP11's Impact on Fusarium TR4 Pathogenicity
作者:Xie, Yanling;Huang, Huoqing;Huo, Yile;Yang, Wenlong;Li, Yuqing;Liu, Siwen;Li, Chunyu;Xie, Yanling;Xie, Yanling;Liu, Siwen;Li, Chunyu;Liu, Siwen;Li, Chunyu
关键词:
Fusarium oxysporum f. sp.cubense tropical race 4; fungal stress adaptation; plant-pathogen interactions; virulence -
Efficient C25-Hydroxylation of Vitamin D3 Utilizing an Artificial Self-Sufficient Whole-Cell Cytochrome P450 Biocatalyst
作者:Liang, Ziqi;Zhou, Qiao;Li, Yicheng;Yang, Xiaojun;Liang, Ziqi;Zhou, Qiao;Li, Yicheng;Liu, Xiaoqing;Shen, Yiwen;Tian, Jian;Wang, Xiaolu;Qin, Xing;Wang, Yuan;Luo, Huiying;Yao, Bin;Tu, Tao
关键词:25-hydroxyvitamin D-3; P450s; self-sufficientP450s; local energetic frustration; linker engineering