Molecular characterization of China rabies virus vaccine strain

文献类型： 外文期刊

第一作者： Jiao, Wenqiang

作者： Jiao, Wenqiang;Yin, Xiangping;Li, Zhiyong;Lan, Xi;Li, Xuerui;Tian, Xiaoting;Li, Baoyu;Yang, Bin;Zhang, Yun;Liu, Jixing

作者机构：

关键词： Rabies virus;aG;China;full-length genome

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2011 年 8 卷

页码：

收录情况： SCI

摘要： Background: Rabies virus (RV), the agent of rabies, can cause a severe encephalomyelitis in several species of mammals, including humans. As a human rabies vaccine strain employed in China, the genetic knowledge of the aG strain has not been fully studied. The main goal of the present study is to amplify the whole genome of aG strain, and genetic relationships between other vaccine strains and wild strains were analyzed. Results: The entire genome of human rabies virus vaccine strain aG employed in China was sequenced; this is the second rabies virus vaccine strain from China to be fully characterized. The overall organization and the length of the genome were similar to that of other lyssaviruses. The length of aG strain was 11925nt, comprising a leader sequence of 58nt, nucleoprotein (N) gene of 1353nt, phosphoprotein (P) gene of 894 nt, matrix protein (M) gene of 609nt, glycoprotein (G) gene of 1575nt, RNA-dependent RNA polymerase (RdRp,L) gene of 6384nt, and a trailer region of 70 nt. There was TGAAAAAAA (TGA(7)) consensus sequence in the end of each gene, except AGA(7) at the end of G gene. There was AACAYYYCT consensus start signal at the beginning of each gene. Conclusions: In this report, we analyzed the full genome of China human rabies vaccine strain aG. Our studies indicated that the genome of aG retained the basic characteristics of RV. At gene level, N was the most conserved among the five coding genes, indicating this gene is the most appropriate for quantitative genotype definition. The phylogenetic analysis of the N indicated the aG strain clustered most closely with Japanese and Russian rabies vaccine strains, suggesting that they may share the same ancestor; also, the aG strain did not share high homology with wild strains isolated from China, making it may not be the best vaccine strain, more research is needed to elucidate the genetic relationship among the RV circulating in China.

分类号：

  • 相关文献

[1]Seroprevalence of Rabies Virus Antibodies in Bats from Southern China. Jiang, Yu,Wang, Lili,Han, Xiaohu,Xia, Xianzhu,Tu, Changchun,Wang, Lili,Zhao, Fuguang,Lu, Zongji,Xuan, Hua. 2010

[2]Full-length genomic characterizations of two canine parvoviruses prevalent in Northwest China. Han, Shi-Chong,Guo, Hui-Chen,Sun, Shi-Qi,Shu, Long,Wei, Yan-Quan,Sun, De-Hui,Liu, Xiang-Tao,Han, Shi-Chong,Guo, Hui-Chen,Sun, Shi-Qi,Shu, Long,Wei, Yan-Quan,Sun, De-Hui,Liu, Xiang-Tao,Shu, Long,Cao, Sui-Zhong,Peng, Guang-Neng. 2015

[3]Chimeric rabies glycoprotein with a transmembrane domain and cytoplasmic tail from Newcastle disease virus fusion protein incorporates into the Newcastle disease virion at reduced levels. Yu, Gui Mei,Zu, Shu Long,Zhou, Wei Wei,Wang, Xi Jun,Shuai, Lei,Wang, Xue Lian,Ge, Jin Ying,Bu, Zhi Gao. 2017

[4]An inactivated recombinant rabies CVS-11 virus expressing two copies of the glycoprotein elicits a higher level of neutralizing antibodies and provides better protection in mice. Xue, Xiang-Hong,Li, Ling,Yang, Song-Tao,Xia, Xian-Zhu,Xue, Xiang-Hong,Zheng, Xue-Xing,Wang, Hua-Lei,Ma, Jin-Zhu,Li, Ling,Gai, Wei-Wei,Wang, Tie-Cheng,Yang, Song-Tao,Xia, Xian-Zhu,Zheng, Xue-Xing,Ma, Jin-Zhu.

[5]Recombinant rabies virus expressing the H protein of canine distemper virus protects dogs from the lethal distemper challenge. Wang, Feng-Xue,Zhang, Shu-Qin,Zhu, Hong-Wei,Yang, Yong,Sun, Na,Tan, Bin,Li, Zhen-Guang,Cheng, Shi-Peng,Wen, Yong-Jun,Li, Zhen-Guang,Fu, Zhen F..

[6]Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice. Yin, Xiangping,Li, Zhiyong,Li, Jiangtao,Zhang, Yun,Li, Xuerui,Li, Baoyu,Yang, Bin,Lan, Xi,Jiao, Wenqiang,Liu, Jixing,Yi, Yongzhu,Li, Yinv,Zhang, Zhifang.

[7]Genetically modified rabies virus ERA strain is safe and induces long-lasting protective immune response in dogs after oral vaccination. Shuai, Lei,Wang, Xijun,Ge, Jinying,Wen, Zhiyuan,Chen, Weiye,Qin, Lide,Bu, Zhigao,Feng, Na,Xia, Xianzhu.

[8]Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production. Tao, Lihong,Ge, Jinying,Wang, Xijun,Wen, Zhiyuan,Zhai, Hongyue,Hua, Tao,Zhao, Bolin,Kong, Dongni,Bu, Zhigao,Yang, Chinglai. 2011

[9]Immunogenicity of multi-epitope-based vaccine candidates administered with the adjuvant Gp96 against rabies. Niu, Yange,Yang, Limin,Qu, Hongren,Li, Jing,Liu, Wenjun,Niu, Yange,Liu, Wenjun,Liu, Ye,Hu, Rongliang,Liu, Ye,Hu, Rongliang,Zhao, Jingyi,Li, Jing. 2016

[10]Immunoenhancement with flagellin as an adjuvant to whole-killed rabies vaccine in mice. Xiao, Xing-xing,Zhang, Yun,Liu, Ji-xing,Wei, Qiao-lin,Yin, Xiang-ping.

[11]Enhancement of blood-brain barrier permeability is required for intravenously administered virus neutralizing antibodies to clear an established rabies virus infection from the brain and prevent the development of rabies in mice. Huang, Chien-Tsun,Li, Zhenguang,Huang, Ying,Zhang, Guoqing,Zhou, Ming,Chai, Qingqing,Fu, Zhen F.,Li, Zhenguang,Wu, Hua,Fu, Zhen F..

[12]Inactivated Recombinant Rabies Viruses Displaying Canine Distemper Virus Glycoproteins Induce Protective Immunity against Both Pathogens. Budaszewski, Renata da Fontoura,Budaszewski, Renata da Fontoura,Sawatsky, Bevan,Kraemer, Beate,von Messling, Veronika,Sawatsky, Bevan,von Messling, Veronika,Hudacek, Andrew,Yin, Xiangping,Schnell, Matthias J.,Yin, Xiangping.

[13]Engineering, expression, and immuno-characterization of recombinant protein comprising multi-neutralization sites of rabies virus glycoprotein. Li, Xiangdong,Luo, Jinyan,Shen, Yang,Qiu, Yafeng,Wang, Xiaodu,Deng, Xufang,Ding, Chan,Ma, Zhiyong,Wang, Shuiming,Liu, Xuehui,Bao, Weidong,Liu, Peihong,Zhou, Jinping.

[14]Characterization of a virulent dog-originated rabies virus affecting more than twenty fallow deer (Dama dama) in Inner Mongolia, China. Zhu, Hongwei,Shao, Xiqun,Ba, Hengxing,Wang, Fengxue,Yang, Yong,Sun, Na,Ren, Jingqiang,Cheng, Shipeng,Wen, Yongjun,Chen, Xiaoyun,Wang, Hualei.

[15]Genetically modified rabies virus-vectored Ebola virus disease vaccines are safe and induce efficacious immune responses in mice and dogs. Shuai, Lei,Wang, Xijun,Wen, Zhiyuan,Ge, Jinying,Wang, Jinliang,Zhao, Dandan,Bu, Zhigao.

[16]Generation of Recombinant Rabies Virus CVS-11 Expressing eGFP Applied to the Rapid Virus Neutralization Test. Xue, Xianghong,Zheng, Xuexing,Liang, Hongru,Feng, Na,Zhao, Yongkun,Gao, Yuwei,Wang, Hualei,Yang, Songtao,Xia, Xianzhu,Xue, Xianghong,Yang, Songtao,Xia, Xianzhu,Zheng, Xuexing,Xia, Xianzhu. 2014

[17]Aptamers targeting rabies virus-infected cells inhibit street rabies virus in vivo. Liang, Hong-Ru,Liang, Hong-Ru,Hu, Gu-Qiu,Li, Ling,Gao, Yu-Wei,Yang, Song-Tao,Xia, Xian-Zhu. 2014

[18]Immunization with recombinant rabies virus expressing Interleukin-18 exhibits enhanced immunogenicity and protection in mice. Gai, Weiwei,Zheng, Wenwen,Song, Yanyan,Gai, Weiwei,Zheng, Xuexing,Wang, Chong,Wong, Gary. 2017

[19]Selection of an aptamer against rabies virus: A new class of molecules with antiviral activity. Liang, Hong-Ru,Liang, Hong-Ru,Hu, Gui-Qiu,Xue, Xiang-Hong,Li, Lu,Zheng, Xue-Xing,Gao, Yu-Wei,Yang, Song-Tao,Xia, Xian-Zhu.

[20]Two potential recombinant rabies vaccines expressing canine parvovirus virion protein 2 induce immunogenicity to canine parvovirus and rabies virus. Luo, Jun,Shi, Hehe,Niu, Xuefeng,Long, Teng,Zhao, Jing,Tian, Qin,Wang, Yifei,Chen, Hao,Guo, Xiaofeng,Luo, Jun,Shi, Hehe,Niu, Xuefeng,Long, Teng,Zhao, Jing,Tian, Qin,Wang, Yifei,Chen, Hao,Guo, Xiaofeng,Tan, Yeping.

作者其他论文 更多>>

微信小程序