Global Identification of Transcription Start Sites in the Genome of Apis mellifera Using 5 ' LongSAGE

文献类型： 外文期刊

第一作者： Zheng, Huajun

作者： Zheng, Huajun;Shen, Yan;Wang, Yuezhu;Gu, Wenyi;Wang, Shengyue;Sun, Liangxian;Chen, Shuting;Huang, Zhouying;Peng, Wenjun;Xu, Baohua

作者机构：

期刊名称：JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION （ 影响因子：2.656； 五年影响因子：2.473 ）

ISSN： 1552-5007

年卷期： 2011 年 316B 卷 7 期

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收录情况： SCI

摘要： The precise identification of the transcription start sites (TSSs) of genes in the honeybee genome will be helpful for inferring start codons and for determining promoter elements. The 5 ' SAGE approach provides a powerful tool for identifying TSSs in the sequenced genome. The main purpose of this study is to identify the actual TSSs of expressed genes as well as the usage of different TSSs in the Apis mellifera genome. We performed a 5 ' LongSAGE (5 ' LS) analysis for the adult drone head, and the TSSs of the expressed genes were determined by mapping the 5 ' LS tag sequences to the honeybee genome. A total of 8,280 unique 19 bp 5 ' LS tag sequences were identified that corresponded to 3,655 predicted genes. Out of these tags, 4,998 tags (60.4%) were mapped to a region from -1,000 bp to + 100 bp of the start codon of 2,301 reference coding sequences. Notably, we observed that 28-47% of the 3,655 honeybee genes initiated transcription from alternative TSSs. The TSS consensus pattern of the honeybee genes, DT(rich)Py (Pu) under bar (G(rich))(T/A)(T(rich))(3), was obtained by aligning the sequences flanking the 5 ' LS-TSSs. We also identified three new genes in the regions downstream of 5 ' LS tags and validated 21 TSSs using RT-PCR amplification. Additionally, 17 genes identified by the 5 ' LS tags were associated with the Gene Ontology term "behavior.'' Mapping of the 5 ' LS tags on the genome not only provided direct evidence of expression for in silico predicted genes but also allowed for the identification of previously unrecognized, novel exons and alternative TSSs. J. Exp. Zool. (Mol. Dev. Evol.) 316:500-514, 2011. (C) 2011 Wiley Periodicals, Inc.

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