Co-existence of multiple strains of porcine circovirus type 2 in the same pig from China

文献类型： 外文期刊

第一作者： Zhai, Shao-Lun

作者： Zhai, Shao-Lun;Chen, Sheng-Nan;Wei, Wen-Kang;Zhai, Shao-Lun;Wei, Zu-Zhang;Zhang, Jian-Wu;Huang, Lv;Lin, Tao;Yuan, Shi-Shan;Long, Jin-Xue;Zhai, Shao-Lun;Chen, Sheng-Nan;Yue, Cheng;Ran, Duo-Liang

作者机构：

关键词： Porcine circovirus type 2;Multiple viral strains;Co-existence;New infection form

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2011 年 8 卷

页码：

收录情况： SCI

摘要： Pigs are often co-infected by different viral strains from the same virus. Up to now, there are few reports about co-existence of different porcine circovirus type 2 (PCV2) strains in China. The aim of this study was to evaluate it in Chinese swine herds. 118 PCV2 positive DNAs isolated from diseased pigs identified by classic PCR were re-detected using a modified differential PCR assay. The results indicated that co-existence rates of PCV2 were 32.2% (38/118) in diseased pigs and 0% (0/41) in asymptomatic pigs. Four PCV2 complete genomes were cloned from two co-infected samples and their nucleotide (nt) identities were 95%-97.3%. The phylogenetic analysis showed that four PCV2 strains were divided into different genotypes, PCV2a, PCV2b, PCV2d and PCV2e, respectively. In addition, co-existence were not detected in 41 serum samples from healthy pigs but PCV2 single infection (31.7%, 13/41) existed. These data revealed that the co-existence of different strains of PCV2 might contribute to the development of more severe clinical symptoms for pigs. This is the first report confirming the co-existence of different PCV2 strains in Chinese swine herds. Meanwhile, this study could help us to understand new infection and prevalence forms of PCV2 clinically.

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