Detection of foot-and-mouth disease virus rna by reverse transcription loop-mediated isothermal amplification

文献类型： 外文期刊

第一作者： Chen, Hao-tai

作者： Chen, Hao-tai;Zhang, Jie;Liu, Yong-sheng;Liu, Xiang-tao

作者机构：

关键词： Foot-and-mouth disease virus;Detection;Reverse transcription loop-mediated isothermal amplification;Sensitivity;Specificity

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2011 年 8 卷

页码：

收录情况： SCI

摘要： A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64 C by employing a set of four primers targeting FMDV 2B. The assay showed higher sensitivity than RT-PCR. No cross reactivity was observed from other RNA viruses including classical swine fever virus, swine vesicular disease, porcine reproductive and respiratory syndrome virus, Japanese encephalitis virus. Furthermore, the assay correctly detected 84 FMDV positive samples but not 65 FMDV negative specimens. The result indicated the potential usefulness of the technique as a simple and rapid procedure for the detection of FMDV infection.

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