Isolation and characterization of EST-SSRs in the Ramie

文献类型： 外文期刊

第一作者： Chen, Jian-Hua

作者： Chen, Jian-Hua;Luan, Ming-Bao;Zou, Zi-Zheng;Wang, Xiao-Fei;Xu, Ying;Sun, Zhi-Min;Song, Shu-Feng

作者机构：

关键词： Microsatellite marker;ramie;EST;EST-SSR

期刊名称：AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH （ 影响因子：0.539； 五年影响因子：0.564 ）

ISSN： 1996-0808

年卷期： 2011 年 5 卷 21 期

页码：

收录情况： SCI

摘要： In order to develop the more EST-SSRs, 320 ESTs of ramie from NCBI were analyzed. 76 SSR primer-pairs developed from 320 ESTs were tested among 62 ramie individuals, and 27 SSR loci were polymorphic. Among the 27 primers, the numbers of alleles per locus ranged from two to five, in which 19 primers amplify two alleles, seven primers amplify three alleles, and only one primer amplifies five alleles. Observed heterozygosity (H(O)) of overall loci among the 27 primer pairs ranged from 0.16 to 0.93 and expected heterozygosity(H(E)) ranged from 0.21 to 0.66. Of the 27 loci, 14 displayed significant deviations from Hardy-Weinberg expectations (P < 0.05). No significant linkage disequilibrium (LD) was detected among the 27 loci. The 27 primers have been used in the ramie molecular linkage map and genetic diversity analysis successfully.

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