Production of gamma-linolenic acid using a novel heterologous expression system in the oleaginous yeast Lipomyces kononenkoae
文献类型： 外文期刊
第一作者： Wang, Ping
作者： Wang, Ping;Wan, Xia;Zhang, Yinbo;Jiang, Mulan;Wan, Xia;Jiang, Mulan
作者机构：
关键词： Delta 6-fatty acid desaturase;gamma-linolenic acid;Lipomyces kononenkoae;Polyunsaturated fatty acid
期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）
ISSN： 0141-5492
年卷期： 2011 年 33 卷 10 期
页码：
收录情况： SCI
摘要： A novel expression system was established in the oleaginous yeast, Lipomyces kononenkoae. The expression vector pLK-rhPHG of L. kononenkoae was constructed and using the hygromycin phosphotransferase gene and green fluorescent protein gene as reporter genes. A delta 6-fatty acid desaturase gene (D6DM) from Cunninghamella echinulata MIAN6 was then expressed in this strain. The recombinant strain accumulated about 1.2% gamma-linolenic acid in the total fatty acids.
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