Comparative proteomic analyses of two Taxus species (Taxus X media and Taxus mairei) reveals variations in the metabolisms associated with paclitaxel and other metabolites

文献类型： 外文期刊

第一作者： Juan Hao

作者： Juan Hao;Shen, Chenjia;Hong Guo;Xinai Shi;Ye Wang;Qinghua Wan;Yao-Bin Song;Lei Zhang;Ming Dong;Chenjia Shen

作者机构：

关键词： flavonoid;LC-MS/MS;proteomic analyses;Taxus;Taxol;TMT labeling

期刊名称：PLANT AND CELL PHYSIOLOGY （ 影响因子：4.927； 五年影响因子：5.516 ）

ISSN： 0032-0781

年卷期： 2017 年 58 卷 11 期

页码：

收录情况： SCI

摘要： Taxus species are well-known for paclitaxel, which exhibits antitumor activities and is used for treating various cancers. Although most Taxus species are widespread in many areas, few studies have characterized the variation in metabolism among different Taxus species. Using an integrated approach involving 'tandem mass tag' labeling and liquid chromatography-tandem mass spectrometry (HPLC-MS), proteomes of T. media and T. mairei were investigated and 4078 proteins were quantified. Screening and classification of differentially expressed proteins revealed many metabolism-associated proteins. In detail, four enzymes involved in the flavonoid biosynthesis pathway were predominantly expressed in T. mairei. Four enzymes associated with supplying precursors for paclitaxel biosynthesis and three cytochrome P450 taxoid oxygenases were preferentially expressed in T. media compared with T. mairei. Furthermore, variations in taxoid contents between T. media and T. mairei were determined using HPLC-MS analysis. Variations in flavonoid contents between T. media and T. mairei were determined by HPLC analysis. A number of differentially expressed proteins may provide an explanation for the variation in metabolisms of different Taxus species.

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