Immunization with recombinant rabies virus expressing Interleukin-18 exhibits enhanced immunogenicity and protection in mice

文献类型: 外文期刊

第一作者: Gai, Weiwei

作者: Gai, Weiwei;Zheng, Wenwen;Song, Yanyan;Gai, Weiwei;Zheng, Xuexing;Wang, Chong;Wong, Gary

作者机构:

关键词: rabies virus;IL-18;immune responses;vaccine;antibody titer

期刊名称:ONCOTARGET ( 影响因子:5.168; 五年影响因子:5.312 )

ISSN: 1949-2553

年卷期: 2017 年 8 卷 53 期

页码:

收录情况: SCI

摘要: Several studies have shown that interleukin-18 (IL-18) plays an important role in both innate and adaptive immune responses. In this study, we investigated the pathogenicity and immunogenicity of recombinant rabies virus expressing IL-18 (rHEP-IL18). Experimental results showed that Institute of Cancer Research (ICR) mice that received a single intramuscular immunization with rHEP-IL18 elicited the highest titers of serum neutralizing antibodies and the strongest cell-mediated immune responses to prevent the development of rabies disease, compared with immunization with the parent virus HEP-Flury. Mice inoculated with rHEP-IL18 developed significantly higher IFN-. responses, increased percentages of CD4(+) and CD8(+) T-lymphocytes compared to HEP-Flury. Flow cytometry results show that rHEPIL18 recruited more activated T-and B-cells in lymph nodes or peripheral blood, which is beneficial for virus clearance in the early stages of infection. A higher percentage of mice immunized with rHEP-IL18 survived wild-type rabies virus (RABV) challenge, compared to HEP-Flury mice. Our results show that rHEP-IL18 is promising as a novel vaccine for RABV prevention and control.

分类号:

  • 相关文献

[1]Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice. Yin, Xiangping,Li, Zhiyong,Li, Jiangtao,Zhang, Yun,Li, Xuerui,Li, Baoyu,Yang, Bin,Lan, Xi,Jiao, Wenqiang,Liu, Jixing,Yi, Yongzhu,Li, Yinv,Zhang, Zhifang.

[2]Two potential recombinant rabies vaccines expressing canine parvovirus virion protein 2 induce immunogenicity to canine parvovirus and rabies virus. Luo, Jun,Shi, Hehe,Niu, Xuefeng,Long, Teng,Zhao, Jing,Tian, Qin,Wang, Yifei,Chen, Hao,Guo, Xiaofeng,Luo, Jun,Shi, Hehe,Niu, Xuefeng,Long, Teng,Zhao, Jing,Tian, Qin,Wang, Yifei,Chen, Hao,Guo, Xiaofeng,Tan, Yeping.

[3]A multivalent killed whole-cell vaccine induces effective protection against Edwardsiella tarda and Vibrio anguillarum. Sun, Yun,Sun, Li,Liu, Chun-sheng,Sun, Yun.

[4]IL-18 enhances protective effect in mice immunized with a Schistosoma japonicum FABP DNA vaccine. Wei, Feng,Liu, Quan,Zhai, Yujia,Liu, Wensen,Shang, Limin,Men, Jingtao,Gao, Shengyan,Lian, Hai,Jin, Hongtao,Chen, Chen,Xia, Zhiping,Fu, Zhiqiang,Lin, Jiaojiao,Shi, Yaojun,Zhu, Xing-Quan,Wei, Feng. 2009

[5]Enhancement by IL-18 of the protective effect of a Schistosoma japonicum 26 kDa GST plasmid DNA vaccine in mice. Wei, Feng,Liu, Quan,Gao, Shengyan,Shang, Limin,Zhai, Yujia,Men, Jingtao,Jiang, Li,Xia, Zhiping,Wei, Feng,Gao, Shengyan,Zhu, Xing-Quan,Fu, Zhiqiang,Shi, Yaojun. 2008

[6]Compound Chinese herbal medicinal ingredients can enhance immune response and efficacy of RHD vaccine in rabbit. Yang, Longsheng,Hu, Yuanliang,Wang, Deyun,Kong, Xiangfeng,Yang, Longsheng,Xue, Jiabin,Wang, Fang,Xu, Weizhong,Li, Peng,Kong, Xiangfeng,Kong, Xiangfeng. 2008

[7]Construction of Swine-Specific CpG Motif Enriched Plasmid and the Study of Its Immunostimulatory Effects Both In Vitro and In Vivo. Guo, Xiaoyu,Jia, Hong,Yuan, Weifeng,Hou, Shaohua,Zhu, Hongfei,Guo, Xiaoyu,Manuel Sanchez-Vizcaino, Jose,Guo, Xiaoyu,Manuel Sanchez-Vizcaino, Jose,Zhang, Quan,Sun, Yao,Zhu, Gang. 2012

[8]Selenylation modification can enhance immune-enhancing activity of Chinese angelica polysaccharide. Qin, Tao,Wang, Deyun,Hu, Yuanliang,Zhang, Jing,Wang, Mi,Qiu, Shulei,Gao, Zhenzhen,Liu, Rongrong,Yu, Yun,Huang, Yee,Wang, Qiaoxiu,Chen, Jin,Wang, Qiaochu.

[9]The Selenylation Modification of Epimedium Polysaccharide and Isatis Root Polysaccharide and the Immune-enhancing Activity Comparison of Their Modifiers. Li, Xiuping,Hou, Ranran,Yue, Chanjuan,Liu, Jie,Gao, Zhenzhen,Wang, Deyun,Liu, Cui,Hu, Yuanliang,Li, Xiuping,Chen, Jin,Lu, Yu.

[10]Comparative study of the immune effect of an Edwardsiella tarda antigen in two forms: Subunit vaccine vs DNA vaccine. Sun, Yun,Sun, Li,Sun, Yun,Liu, Chun-Sheng.

[11]Chimeric rabies glycoprotein with a transmembrane domain and cytoplasmic tail from Newcastle disease virus fusion protein incorporates into the Newcastle disease virion at reduced levels. Yu, Gui Mei,Zu, Shu Long,Zhou, Wei Wei,Wang, Xi Jun,Shuai, Lei,Wang, Xue Lian,Ge, Jin Ying,Bu, Zhi Gao. 2017

[12]Seroprevalence of Rabies Virus Antibodies in Bats from Southern China. Jiang, Yu,Wang, Lili,Han, Xiaohu,Xia, Xianzhu,Tu, Changchun,Wang, Lili,Zhao, Fuguang,Lu, Zongji,Xuan, Hua. 2010

[13]An inactivated recombinant rabies CVS-11 virus expressing two copies of the glycoprotein elicits a higher level of neutralizing antibodies and provides better protection in mice. Xue, Xiang-Hong,Li, Ling,Yang, Song-Tao,Xia, Xian-Zhu,Xue, Xiang-Hong,Zheng, Xue-Xing,Wang, Hua-Lei,Ma, Jin-Zhu,Li, Ling,Gai, Wei-Wei,Wang, Tie-Cheng,Yang, Song-Tao,Xia, Xian-Zhu,Zheng, Xue-Xing,Ma, Jin-Zhu.

[14]Recombinant rabies virus expressing the H protein of canine distemper virus protects dogs from the lethal distemper challenge. Wang, Feng-Xue,Zhang, Shu-Qin,Zhu, Hong-Wei,Yang, Yong,Sun, Na,Tan, Bin,Li, Zhen-Guang,Cheng, Shi-Peng,Wen, Yong-Jun,Li, Zhen-Guang,Fu, Zhen F..

[15]Genetically modified rabies virus ERA strain is safe and induces long-lasting protective immune response in dogs after oral vaccination. Shuai, Lei,Wang, Xijun,Ge, Jinying,Wen, Zhiyuan,Chen, Weiye,Qin, Lide,Bu, Zhigao,Feng, Na,Xia, Xianzhu.

[16]Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production. Tao, Lihong,Ge, Jinying,Wang, Xijun,Wen, Zhiyuan,Zhai, Hongyue,Hua, Tao,Zhao, Bolin,Kong, Dongni,Bu, Zhigao,Yang, Chinglai. 2011

[17]Immunogenicity of multi-epitope-based vaccine candidates administered with the adjuvant Gp96 against rabies. Niu, Yange,Yang, Limin,Qu, Hongren,Li, Jing,Liu, Wenjun,Niu, Yange,Liu, Wenjun,Liu, Ye,Hu, Rongliang,Liu, Ye,Hu, Rongliang,Zhao, Jingyi,Li, Jing. 2016

[18]Immunoenhancement with flagellin as an adjuvant to whole-killed rabies vaccine in mice. Xiao, Xing-xing,Zhang, Yun,Liu, Ji-xing,Wei, Qiao-lin,Yin, Xiang-ping.

[19]Enhancement of blood-brain barrier permeability is required for intravenously administered virus neutralizing antibodies to clear an established rabies virus infection from the brain and prevent the development of rabies in mice. Huang, Chien-Tsun,Li, Zhenguang,Huang, Ying,Zhang, Guoqing,Zhou, Ming,Chai, Qingqing,Fu, Zhen F.,Li, Zhenguang,Wu, Hua,Fu, Zhen F..

[20]Inactivated Recombinant Rabies Viruses Displaying Canine Distemper Virus Glycoproteins Induce Protective Immunity against Both Pathogens. Budaszewski, Renata da Fontoura,Budaszewski, Renata da Fontoura,Sawatsky, Bevan,Kraemer, Beate,von Messling, Veronika,Sawatsky, Bevan,von Messling, Veronika,Hudacek, Andrew,Yin, Xiangping,Schnell, Matthias J.,Yin, Xiangping.

作者其他论文 更多>>