Immunization with recombinant rabies virus expressing Interleukin-18 exhibits enhanced immunogenicity and protection in mice
文献类型: 外文期刊
第一作者: Gai, Weiwei
作者: Gai, Weiwei;Zheng, Wenwen;Song, Yanyan;Gai, Weiwei;Zheng, Xuexing;Wang, Chong;Wong, Gary
作者机构:
关键词: rabies virus;IL-18;immune responses;vaccine;antibody titer
期刊名称:ONCOTARGET ( 影响因子:5.168; 五年影响因子:5.312 )
ISSN: 1949-2553
年卷期: 2017 年 8 卷 53 期
页码:
收录情况: SCI
摘要: Several studies have shown that interleukin-18 (IL-18) plays an important role in both innate and adaptive immune responses. In this study, we investigated the pathogenicity and immunogenicity of recombinant rabies virus expressing IL-18 (rHEP-IL18). Experimental results showed that Institute of Cancer Research (ICR) mice that received a single intramuscular immunization with rHEP-IL18 elicited the highest titers of serum neutralizing antibodies and the strongest cell-mediated immune responses to prevent the development of rabies disease, compared with immunization with the parent virus HEP-Flury. Mice inoculated with rHEP-IL18 developed significantly higher IFN-. responses, increased percentages of CD4(+) and CD8(+) T-lymphocytes compared to HEP-Flury. Flow cytometry results show that rHEPIL18 recruited more activated T-and B-cells in lymph nodes or peripheral blood, which is beneficial for virus clearance in the early stages of infection. A higher percentage of mice immunized with rHEP-IL18 survived wild-type rabies virus (RABV) challenge, compared to HEP-Flury mice. Our results show that rHEP-IL18 is promising as a novel vaccine for RABV prevention and control.
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