Characterization of oqxAB in Escherichia coli Isolates from Animals, Retail Meat, and Human Patients in Guangzhou, China

文献类型： 外文期刊

第一作者： Wang, Jing

作者： Wang, Jing;Zhi, Chan-Ping;Chen, Xiao-Jie;Guo, Ze-Wen;Liu, Wu-Ling;Luo, Juan;Huang, Xin-Yi;Zeng, Li;Huang, Jia-Wei;Xia, Ying-Bi;Yi, Meng-Ying;Huang, Teng;Zeng, Zhen-Ling;Liu, Jian-Hua;Chen, Xiao-Jie;Liu, Wu-Ling;Zeng, Li

作者机构：

关键词： antimicrobial resistance;Escherichia coli;food safety;plasmids;PMQR

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： The purpose of this study was to investigate the prevalence and genetic elements of oqxAB among Escherichia coli isolates from animals, retail meat, and humans (patients with infection or colonization) in Guangzhou, China. A total of 1,354 E. coli isolates were screened for oqxAB by PCR. Fifty oqxAB-positive isolates were further characterized by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), S1-PFGE, genetic environment analysis, plasmid replicon typing, and plasmid sequencing. oqxAB was detected in 172 (33.79%), 60 (17.34%), and 90 (18.07%) E. coli isolates from animal, food, and human, respectively. High clonal diversity was observed among oqxAB-positive isolates. In 21 oqxAB-containing transformants, oqxAB was flanked by two IS26 elements in the same orientation, formed a composite transposon Tn6010 in 19 transformants, and was located on plasmids (33.3 similar to 500 kb) belonging to IncN1-F33:A-:B- (n = 3), IncHI2/ST3 (n = 3), F-:A18:B- (n = 2), F-:A-:B54 (n = 2), or others. Additionally, oqxAB was co-located with multiple resistance genes on the same plasmid, such as aac(6')-Ib-cr and/or qnrS, which were identified in two F-:A18:B- plasmids from pigs, and bla(CTX-M-55), rmtB, fosA3, and floR, which were detected in two N1-F33:A-:B- plasmids from patients. The two IncHI2/ST3 oqxAB-bearing plasmids, pHNLDF400 and pHNYJC8, which were isolated from human patient and chicken meat, respectively, contained a typical IncHI2-type backbone, and were similar to each other with 2-bp difference, and also showed 99% identity to the Salmonella Typhimurium oqxAB-carrying plasmids pHXY0908 (chicken) and pHK0653 (human patient). Horizontal transfer mediated by mobile elements may be the primary mechanism underlying oqxAB spread in E. coli isolates obtained from various sources in Guangzhou, China. The transmission of identical oqxAB-carrying IncHI2 plasmids between food products and humans might pose a serious threat to public health.

分类号：

  • 相关文献

[1]Resistance of Strains Producing Extended-Spectrum beta-lactamases and Genotype Distribution Among Escherichia coli in China. Xue, Yuan,Hua, Yuping,Chen, Jianfei. 2012

[2]CHARACTERIZATION OF INTEGRON-MEDIATED ANTIMICROBIAL RESISTANCE AMONG ESCHERICHIA COLI STRAINS ISOLATED FROM A CAPTIVE POPULATION OF AMUR TIGERS IN CHINA. Xue, Yuan,Wang, Yulong,Zhang, Yanlong,Hua, Yuping,Chen, Jianfei,Liu, Dan. 2013

[3]Morphology analysis of Escherichia coli treated with nonthermal plasma. Guo, J.,Li, Z.,Huang, K.,Li, Y.,Wang, J.,Guo, J.,Li, Y..

[4]Development of a replicative plasmid for gene expression in Mycoplasma bovis. Li, Jiahe,Zhang, Ning,Zhang, Yuewei,Wu, Wenxue,Zhang, Jixiang,Li, Jinxiang.

[5]Identification of a novel vga(E) gene variant that confers resistance to pleuromutilins, lincosamides and streptogramin A antibiotics in staphylococci of porcine origin. Li, Jun,Wang, Yang,Wu, Congming,Shen, Jianzhong,Li, Beibei,Ma, Zhiyong,Wendlandt, Sarah,Schwarz, Stefan.

[6]Detection and new genetic environment of the pleuromutilinlincosamidestreptogramin A resistance gene lsa(E) in methicillin-resistant Staphylococcus aureus of swine origin. Li, Beibei,Yao, Jiannan,Shi, Zixue,Wei, Jianchao,Shao, Donghua,Wang, Shaohui,Ma, Zhiyong,Wendlandt, Sarah,Schwarz, Stefan,Liu, Yiqiu,Zhang, Qing. 2013

[7]Construction of pTM series plasmids for gene expression in Brucella species. Tian, Mingxing,Qu, Jing,Bao, Yanqing,Gao, Jianpeng,Liu, Jiameng,Wang, Shaohui,Sun, Yingjie,Ding, Chan,Yu, Shengqing.

[8]Identification of the novel spectinomycin resistance gene spw in methicillin-resistant and methicillin-susceptible Staphylococcus aureus of human and animal origin. Wendlandt, Sarah,Li, Beibei,Lozano, Carmen,Ma, Zhiyong,Torres, Carmen,Schwarz, Stefan. 2013

[9]Plasmid-borne cadmium resistant determinants are associated with the susceptibility of Listeria monocytogenes to bacteriophage. Zhang, Hui,Zhou, Yan,Bao, Hongduo,Zhang, Lili,Wang, Ran,Zhang, Hui,Zhou, Xiaohui.

[10]Antimicrobial Resistance of Campylobacter Species Isolated from Broilers in Live Bird Markets in Shanghai, China. Li, Beibei,Li, Yingli,Jia, Haiyan,Wei, Jianchao,Shao, Donghua,Liu, Ke,Shi, Yuanyuan,Qiu, Yafeng,Ma, Zhiyong,Ma, Licai. 2017

[11]Characterization of antimicrobial resistance of Vibrio parahaemolyticus from cultured sea cucumbers (Apostichopus japonicas). Jiang, Y.,Yao, L.,Li, F.,Tan, Z.,Zhai, Y.,Wang, L..

[12]Genetic characterization of antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis cases in Northwest China. Yang Feng,Wang Xu-rong,Wang Ling,Li Xin-pu,Luo Jin-yin,Zhang Shi-dong,Li Hong-sheng,Wang Qi. 2016

[13]Occurrence and antimicrobial susceptibility of Listeria monocytogenes isolates from retail raw foods. Wang, Xiu-Mei,Liu, Hui-Fang,Zhang, Wan-Jiang,Si, Wei,Yu, Shen-Ye,Liu, Si-Guo,Lu, Xing-Feng,Yin, Lu,Shao, Mei-Li. 2013

[14]Prevalence, antimicrobial susceptibility, and molecular characterization of Staphylococcus aureus isolated from dairy herds in northern China. Liu, Huimin,Li, Songli,Meng, Lu,Dong, Lei,Zhao, Shengguo,Lan, Xinyi,Wang, Jiaqi,Zheng, Nan,Liu, Huimin,Li, Songli,Meng, Lu,Dong, Lei,Zhao, Shengguo,Lan, Xinyi,Wang, Jiaqi,Zheng, Nan. 2017

[15]Resistance integrons: class 1, 2 and 3 integrons. Deng, Yang,Bao, Xuerui,Ji, Lili,Liu, Junyan,Miao, Jian,Chen, Lei,Chen, Dingqiang,Bian, Huawei,Li, Yanmei,Yu, Guangchao. 2015

[16]Multidrug resistance genes in staphylococci from animals that confer resistance to critically and highly important antimicrobial agents in human medicine. Wendlandt, Sarah,Kadlec, Kristina,Fessler, Andrea T.,Schwarz, Stefan,Shen, Jianzhong,Wang, Yang,Wu, Congming,Li, Beibei,Zhang, Wan-Jiang.

[17]Antimicrobial resistance and toxin gene profiles of Staphylococcus aureus strains from Holstein milk. Wang, X.,Wang, Y.,Usman, T.,Zhang, Y.,Yu, Y.,Wang, X.,Hao, D.,Tang, X.,Guo, G.,Guo, G.. 2014

[18]Molecular epidemiology, virulence determinants and antimicrobial resistance of Campylobacter spreading in retail chicken meat in Central China. Zhang, Tengfei,Luo, Qingping,Chen, Yiluo,Wen, Guoyuan,Zhang, Rongrong,Luo, Ling,Lu, Qin,Ai, Diyun,Wang, Hongcai,Shao, Huabin,Chen, Yiluo,Li, Tingting. 2016

[19]Prevalence, Enterotoxin Gene and Antimicrobial Resistance of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus from Clinical Healthy Dairy Cows. Bao, Hongduo,Zhang, Hui,Zhou, Yan,Zhang, Lili,Wang, Ran.

[20]Characterization of extended-spectrum beta-lactamases (ESBLs)-producing Salmonella in retail raw chicken carcasses. Qiao, Jing,Zhang, Qiang,Wang, Jiawei,Meng, Lingyuan,Yang, Baowei,Alali, Walid Q.,Xiao, Yingping,Yang, Hua,Xiao, Yingping,Yang, Hua,Chen, Sheng,Cui, Shenghui.

作者其他论文 更多>>

微信小程序