Detection and phylogenetic analyses of spike genes in porcine epidemic diarrhea virus strains circulating in China in 2016-2017

文献类型： 外文期刊

第一作者： Zhang, Qiaoling

作者： Zhang, Qiaoling;Liu, Xinsheng;Fang, Yuzhen;Zhou, Peng;Wang, Yonglu;Zhang, Yongguang;Zhang, Qiaoling;Liu, Xinsheng;Fang, Yuzhen;Zhou, Peng;Wang, Yonglu;Zhang, Yongguang

作者机构：

关键词： Pedv;Spike gene;Phylogenetic analysis;China

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2017 年 14 卷

页码：

收录情况： SCI

摘要： Background: Large-scale outbreaks of porcine epidemic diarrhea (PED) have re-emerged in China in recent years. However, little is known about the genetic diversity and molecular epidemiology of field strains of PED virus (PEDV) in China in 2016-2017. To address this issue, in this study, 116 diarrhea samples were collected from pig farms in 6 Chinese provinces in 2016-2017 and were detected using PCR for main porcine enteric pathogens, including PEDV, porcine deltacoronavirus (PDCoV), porcine transmissible gastroenteritis virus (TGEV) and porcine kobuvirus (PKV). In addition, the complete S genes from 11 representative PEDV strains were sequenced and analyzed. Results: PCR detection showed that 52.6% (61/116) of these samples were positive for PEDV. Furthermore, sequencing results for the spike (S) genes from 11 of the epidemic PEDV strains showed 93-94% nucleotide identity and 92-93% amino acid identity with the classical CV777 strain. Compared with the CV777 vaccine strain, these strains had an insertion (A(133)), a deletion (G(155)), and a continuous 4-amino-acid insertion ((NNTN59)-N-56) in the S1 region. Phylogenetic analysis based on the S gene indicated that the 11 assessed PEDV strains were genetically diverse and clustered into the G2 group. These results demonstrate that the epidemic strains of PEDV in China in 2016-2017 are mainly virulent strains that belong to the G2 group and genetically differ from the vaccine strain. Importantly, this is the first report that the samples collected in Hainan Province were positive for PEDV (59.2%, 25/42). Conclusions: To our knowledge, this article presents the first report of a virulent PEDV strain isolated from Hainan Island, China. The results of this study will contribute to the understanding of the epidemiology and genetic characteristics of PEDV in China.

分类号：

  • 相关文献

[1]Detection and phylogenetic analysis of porcine epidemic diarrhea virus in central China based on the ORF3 gene and the S1 gene. Su, Yunfang,Ji, Pengchao,Liu, Yunchao,Chen, Yumei,Xing, Guangxu,Liu, Chang,Deng, Ruiguang,Zhang, Gaiping,Zhao, Baolei,Jiang, Dawei,Song, Yapeng,Wang, Guoqiang,Li, Dongliang,Zhang, Gaiping,Zhang, Gaiping. 2016

[2]Sequence Heterogeneity of the ORF3 Gene of Porcine Epidemic Diarrhea Viruses Field Samples in Fujian, China, 2010-2012. Chen, Xi,Yang, Jinxian,Yu, Fusong,Ge, Junqing,Gao, Xindang,Song, Tieying,Zeng, Lili,Guo, Qing. 2013

[3]Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) samples from field cases in Fujian, China. Chen, Xi,Yang, Jinxian,Yu, Fusong,Ge, Junqing,Lin, Tianlong,Song, Tieying. 2012

[4]Human Infection with Orf Virus from Goats in China, 2012. Zhang, Keshan,Liu, Yongjie,Kong, Hanjin,Shang, Youjun,Liu, Xiangtao. 2014

[5]Molecular characteristics and evolutionary analysis of field Marek's disease virus prevalent in vaccinated chicken flocks in recent years in China. Yu, Zu-Hua,Chi, Jia-Qi,Hu, Bo,Liu, Ju-Xiong,Yu, Zu-Hua,Teng, Man,Luo, Jun,Yu, Le-Le,Su, Jing-Wei,Chi, Jia-Qi,Zhao, Pu,Hu, Bo,Zhang, Gai-Ping,Yu, Zu-Hua,Ding, Ke,Luo, Jun,Wang, Xin-Wei,Su, Jing-Wei.

[6]Diagnosis and phylogenetic analysis of ovine pulmonary adenocarcinoma in China. Zhang, Keshan,Liu, Yongjie,Shang, Youjun,Liu, Xiangtao,Kong, Hanjin,Wu, Bin. 2014

[7]Molecular, Biological and Phylogenetic Analysis of Chinese Isolates of Hop stunt viroid Associated with Citrus Cachexia Disease. Wang, Xuefeng,Zhou, Yan,Li, Zhongan,Tang, Kezhi,Liu, Yongqing,Cao, Mengji,Zhou, Changyong,Wang, Xuefeng,Liu, Yongqing,Cao, Mengji.

[8]Phylogenetic relationships between Dicrocoelium chinensis populations in Japan and China based on mitochondrial nad1 gene sequences. Hayashi, Kei,Ohari, Yuma,Ohtori, Maiko,Mohanta, Uday Kumar,Itagaki, Tadashi,Hayashi, Kei,Ohari, Yuma,Mohanta, Uday Kumar,Itagaki, Tadashi,Tang, WenQiang,Matsuo, Kayoko,Matsuo, Kayoko,Sato, Hiroshi.

[9]Molecular characterization of Japanese encephalitis viruses circulating in pigs and mosquitoes on pig farms in the Chinese province of Henan. Teng, Man,Luo, Jun,Wang, Xing-Tao,Zhang, Gai-Ping,Fan, Jian-Ming,Yao, Wu,Chen, Lu,Wang, Chuan-Qing.

[10]Complete genomic characterization of a porcine reproductive and respiratory syndrome virus isolate in Xinjiang province of China. Xia, Jun,Du, Wei,Liu, Yanfeng,Yan, Genqiang,Xia, Jun,Chen, Shijun,Huang, Jion,Ma, Wenge,Yang, Xueyun.

[11]A new variant of rabbit hemorrhagic disease virus G2-like strain isolated in China. Hu, Bo,Fan, Zhiyu,Wang, Fang,Song, Yanhua,Wei, Houjun,Liu, Xing,Qiu, Rulong,Xu, Weizhong,Xue, Jiabin,Hu, Bo,Fan, Zhiyu,Wang, Fang,Song, Yanhua,Wei, Houjun,Liu, Xing,Qiu, Rulong,Xu, Weizhong,Xue, Jiabin,Yuan, Wanzhe.

[12]Occurrence and sequence analysis of porcine deltacoronaviruses in southern China. Zhai, Shao-Lun,Wei, Wen-Kang,Li, Xiao-Peng,Wen, Xiao-Hui,Zhou, Xia,Zhang, He,Lv, Dian-Hong,Li, Feng,Wang, Dan,Li, Feng. 2016

[13]Reverse transcription-PCR assays for the differentiation of various US porcine epidemic diarrhea virus strains. Liu, Xinsheng,Wang, Qiuhong,Liu, Xinsheng.

[14]Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus. Yu, Xuewu,Shi, Lin,Lv, Xiaoping,Zheng, Shimin,Yu, Xuewu,Shi, Lin,Yao, Wei,Cao, Minghui,Yu, Hanxun,Wang, Xiurong,Wang, Xiurong. 2015

[15]PEDV ORF3 encodes an ion channel protein and regulates virus production. Wang, Kai,Lu, Wei,Xie, Shiqi,Yu, Wenjing,Feng, Li,Sun, Bing,Bian, Chao,Sun, Bing,Chen, Jianfei,Shi, Hongyan,Feng, Li,Hsu, Haojen,Fischer, Wolfgang B.,Schwarz, Wolfgang,Schwarz, Wolfgang.

[16]Tight Junction Protein Occludin Is a Porcine Epidemic Diarrhea Virus Entry Factor. Luo, Xiaolei,Guo, Longjun,Zhang, Jian,Xu, Yunfei,Gu, Weihong,Feng, Li,Wang, Yue.

[17]Outbreak of Porcine Epidemic Diarrhea in Piglets in Gansu Province, China. Wang, Hui,Xia, Xinchao,Liu, Yongming,Wang, Shengyi,Qi, Zhiming,Liu, Shixiang,Wang, Haijun,Liu, Zhiqi,Niu, Xu,Liu, Shanshan. 2013

[18]Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China. Chen, Jianfei,Liu, Xiaozhen,Shi, Da,Shi, Hongyan,Zhang, Xin,Li, Changlong,Chi, Yanbin,Feng, Li. 2013

[19]A novel duplex TaqMan probe-based real-time RT-qPCR for detecting and differentiating classical and variant porcine epidemic diarrhea viruses. Su, Yunfang,Su, Yunfang,Liu, Yunchao,Chen, Yumei,Xing, Guangxu,Hao, Huifang,Wei, Qiang,Liang, Yue,Xie, Weitao,Deng, Ruiguang,Zhang, Gaiping,Li, Dongliang,Huang, Huimin,Zhang, Gaiping,Zhang, Gaiping. 2018

[20]Cell Attachment Domains of the Porcine Epidemic Diarrhea Virus Spike Protein Are Key Targets of Neutralizing Antibodies. Li, Chunhua,Li, Chunhua,Li, Wentao,de Esesarte, Eduardo Lucio,Guo, Hongbo,Rottier, Peter J. M.,Bosch, Berend-Jan,van den Elzen, Paul,Aarts, Eduard,van den Born, Erwin.

作者其他论文 更多>>

微信小程序