Integration of lncRNA-miRNA-mRNA reveals novel insights into oviposition regulation in honey bees

文献类型： 外文期刊

第一作者： Chen, Xiao

作者： Chen, Xiao;Chen, Chao;Shi, Wei;Liu, Zhiguang;Wang, Huihua;Guo, Haikun;Ma, Ce;Lu, Qian

作者机构：

关键词： Oviposition;lncRNA;miRNA;mRNA;Honey bee queens;Ovary activation

期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）

ISSN： 2167-8359

年卷期： 2017 年 5 卷

页码：

收录情况： SCI

摘要： Background. The honey bee (Apis mellifera) is a highly diverse species commonly used for honey production and pollination services. The oviposition of the honey bee queen affects the development and overall performance of the colony. To investigate the ovary activation and oviposition processes on a molecular level, a genome-wide analysis of lncRNAs, miRNAs and mRNA expression in the ovaries of the queens was performed to screen for differentially expressed coding and noncoding RNAs. Further analysis identified relevant candidate genes or RNAs. Results. The analysis of the RNA profiles in different oviposition phase of the queens revealed that 740 lncRNAs, 81 miRNAs and 5,481 mRNAs were differently expressed during the ovary activation; 88 lncRNAs, 13 mRNAs and 338 mRNAs were differently expressed during the oviposition inhibition process; and finally, 100 lncRNAs, four miRNAs and 497 mRNAs were differently expressed during the oviposition recovery process. In addition, functional annotation of differentially expressed RNAs revealed several pathways that are closely related to oviposition, including hippo, MAPK, notch, Writ, mTOR, TGF-beta and FoxO signaling pathways. Furthermore, in the QTL region for ovary size, 73 differentially expressed genes and 14 differentially expressed lncRNAs were located, which are considered as candidate genes affecting ovary size and oviposition. Moreover, a core set of genes served as bridges among different miRNAs were identified through the integrated analysis of lncRNA-miRNA-mRNA network. Conclusion. The observed dramatic expression changes of coding and noncoding RNAs suggest that they may play a critical role in honey bee queens' oviposition. The identified candidate genes for oviposition activation and regulation could serve as a resource for further studies of genetic markers of oviposition in honey bees.

分类号：

  • 相关文献

[1]Dissection of Myogenic Differentiation Signatures in Chickens by RNA-Seq Analysis. Li, Tingting,Zhang, Genxi,Wu, Pengfei,Liu, Qiuhong,Wang, Jinyu,Duan, Lian,Li, Guohui. 2018

[2]Transcriptomic Analysis of Long Non-Coding RNAs and Coding Genes Uncovers a Complex Regulatory Network That Is Involved in Maize Seed Development. Zhu, Ming,Jiang, Haiyang,Zhu, Ming,Zhang, Min,Xing, Lijuan,Li, Wenzong,Wang, Lei,Xu, Miaoyun. 2017

[3]An Integrated Analysis of miRNAs and Methylated Genes Encoding mRNAs and lncRNAs in Sheep Breeds with Different Fecundity. Miao, Xiangyang,Luo, Qingmiao,Zhao, Huijing,Qin, Xiaoyu. 2017

[4]Dissecting the biological relationship between TCGA miRNA and mRNA sequencing data using MMiRNA-Viewer. Bai, Yongsheng,Ding, Lizhong,Rath, Ethan,Stuart, Gary,Bai, Yongsheng,Stuart, Gary,Baker, Steve,Jiang, Feng,Bai, Jenny M.,Wu, Jianghong,Jiang, Hui. 2016

[5]Genome-wide transcriptome analysis of mRNAs and microRNAs in Dorset and Small Tail Han sheep to explore the regulation of fecundity. Miao, Xiangyang,Qingmiao Luo Xiaoyu Qin. 2015

[6]Genome-wide analysis reveals the differential regulations of mRNAs and miRNAs in Dorset and Small Tail Han sheep muscles. Miao, Xiangyang,Luo, Qingmiao,Qin, Xiaoyu.

[7]Reproductive traits and mandibular gland pheromone of anarchistic honey bee workers Apis mellifera occurring in China. Niu, De-Fang,Zheng, Huo-Qing,Ping, Shun,Hu, Fu-Liang,Niu, De-Fang,Pirk, Christian W. W.,Shi, Jin-Hu,Cao, Lian-Fei.

[8]Identification and characterization of long non-coding RNAs in subcutaneous adipose tissue from castrated and intact full-sib pair Huainan male pigs. Wang, Jing,Hua, Liushuai,Chen, Junfeng,Zhang, Jiaqing,Bai, Xianxiao,Gao, Binwen,Shi, Zhihai,Sheng, Weidong,Xing, Baosong,Li, Congjun,Gao, Yuan. 2017

[9]Differential regulation of mRNAs and lncRNAs related to lipid metabolism in two pig breeds. Huang, Wanlong,Zhang, Xiuxiu,Li, Ai,Xie, Lingli,Miao, Xiangyang. 2017

[10]Long non-coding RNA analysis of muscular responses to testosterone deficiency in Huainan male pigs. Xing, Baosong,Bai, Xianxiao,Guo, Hongxia,Chen, Junfeng,Hua, Liushuai,Zhang, Jiaqing,Ma, Qiang,Ren, Qiaoling,Wang, Jing,Wang, Huashuai.

[11]Discovery of novel long non-coding RNAs induced by subgroup J avian leukosis virus infection in chicken. Qiu, Lingling,Li, Zhiteng,Chang, Guobin,Bi, Yulin,Xu, Lu,Zhang, Yang,Zhao, Wenming,Xu, Qi,Chen, Guohong,Liu, Xiangping.

[12]Divergently expressed gene identification and interaction prediction of long noncoding RNA and mRNA involved in duck reproduction. Ren, Jindong,Hu, Jianhong,Ren, Jindong,Du, Xue,Zeng, Tao,Chen, Li,Shen, Junda,Lu, Lizhi.

[13]Fitness of Encarsia sophia (Hymenoptera: Aphelinidae) parasitizing Trialeurodes vaporariorum and Bemisia tabaci (Hemiptera: Aleyrodidae). Luo, Chen,Liu, Tong-Xian,Luo, Chen. 2011

[14]Variation of pre-ovipositional period in the brown planthopper, Nilaparvata lugens, collected in tropical, subtropical and temperate Asia. Wada, T.,Ito, K.,Takahashi, A.,Tang, J.. 2007

[15]Host Status of Wheat and Corn for Chilo suppressalis (Lepidoptera: Crambidae). Hao, Lixia,Han, Yongqiang,Hao, Lixia,Liao, Xiaolan.

[16]The immunomodulatory protein RH36 is relating to blood-feeding success and oviposition in hard ticks. Wang, Fangfang,Lu, Xiaojuan,Guo, Fengxun,Gong, Haiyan,Zhang, Houshuang,Zhou, Yongzhi,Cao, Jie,Zhou, Jinlin,Zhou, Jinlin.

[17]Mycangial fungus benefits the development of a leaf-rolling weevil, Euops chinesis. Ding, Jianqing,Li, Xiaoqiong,Guo, Wenfeng.

[18]Electrophysiological Responses and Reproductive Behavior of Fall Webworm Moths (Hyphantria cunea Drury) are Influenced by Volatile Compounds from Its Mulberry Host (Morus alba L.). Tang, Rui,Zhang, Zhong-Ning,Tang, Rui,Zhang, Feng,Tang, Rui,Zhang, Feng. 2016

[19]Effects of mating on ovarian development and oviposition of Apolygus lucorum. Yuan, Wei,Li, Yunhe,Wu, Kongming,Zhao, Xincheng. 2017

[20]Emergence, Mating and Oviposition Behavior of the Chinese Population in Pink Bollworm, Pectinophora gossypiella (Lepidoptera: Gelechiidae). Wi Huai-heng,Wu Kong-ming,Wi Huai-heng,Huang Min-song,Wan Peng,Wyckhuys, Kris A. G.. 2013

作者其他论文 更多>>

微信小程序