Caucasian clover (Trifolium ambiguum Bieb.) x white clover (T. repens L.) - interspecific hybrids developed through tissue culture

文献类型： 外文期刊

第一作者： Huang, Fan

作者： Huang, Fan;Wang, Mingjiu;Li, Zhiyong;Huang, Fan;Wang, Mingjiu

作者机构：

关键词： Caucasian clover;Embryo rescue;ISSR markers;Tissue culture;White clover;Interspecific hybrid

期刊名称：LEGUME RESEARCH （ 影响因子：0.589； 五年影响因子：0.648 ）

ISSN： 0250-5371

年卷期： 2017 年 40 卷 5 期

页码：

收录情况： SCI

摘要： In order to create new forage variety, caucasian clover (Trifolium ambiguum Bieb.) with strong tolerance to drought and cold introduced from New Zealand as the female parent was hybridized with a white clover (T. repens L.) collected from northeast China, which has strong nitrogen fixation capacity. Hybrid embryos of caucasian cloverxwhite clover were successfully raised using embryo rescue. 13.8% of these embryos grew to maturity. The successfully grown plants were transferred to sterilized vermiculite after 5-6 weeks and then to field after 3-4 months. The hybrid plant showed intermediate morphological features and a chromosomal number of 2n=5X=40. In order to obtain more plants for backcross, a tissue culture system was established for the hybrids, using MS+0.1mg l(-1) 2, 4-D+2mg l(-1) 6-BA, MS +0.5mg l(-1) NAA+1mg l(-1) 6-BA+1mg l(-1) KT and 1/2MS as the medium for bud-induction, differentiation and root-induction respectively. 80% of the cultured plantlets survived these growing conditions. Hybridity of the plant was also confirmed by ISSR markers using 16 primers.

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