Caucasian clover (Trifolium ambiguum Bieb.) x white clover (T. repens L.) - interspecific hybrids developed through tissue culture
文献类型: 外文期刊
第一作者: Huang, Fan
作者: Huang, Fan;Wang, Mingjiu;Li, Zhiyong;Huang, Fan;Wang, Mingjiu
作者机构:
关键词: Caucasian clover;Embryo rescue;ISSR markers;Tissue culture;White clover;Interspecific hybrid
期刊名称:LEGUME RESEARCH ( 影响因子:0.589; 五年影响因子:0.648 )
ISSN: 0250-5371
年卷期: 2017 年 40 卷 5 期
页码:
收录情况: SCI
摘要: In order to create new forage variety, caucasian clover (Trifolium ambiguum Bieb.) with strong tolerance to drought and cold introduced from New Zealand as the female parent was hybridized with a white clover (T. repens L.) collected from northeast China, which has strong nitrogen fixation capacity. Hybrid embryos of caucasian cloverxwhite clover were successfully raised using embryo rescue. 13.8% of these embryos grew to maturity. The successfully grown plants were transferred to sterilized vermiculite after 5-6 weeks and then to field after 3-4 months. The hybrid plant showed intermediate morphological features and a chromosomal number of 2n=5X=40. In order to obtain more plants for backcross, a tissue culture system was established for the hybrids, using MS+0.1mg l(-1) 2, 4-D+2mg l(-1) 6-BA, MS +0.5mg l(-1) NAA+1mg l(-1) 6-BA+1mg l(-1) KT and 1/2MS as the medium for bud-induction, differentiation and root-induction respectively. 80% of the cultured plantlets survived these growing conditions. Hybridity of the plant was also confirmed by ISSR markers using 16 primers.
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